TIAR (D32D3) XP? Rabbit mAb

• 产品编号：CST-8509P      品牌：Cell Signaling Technology       原厂货号：8509P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TIAR (D32D3) XP® Rabbit mAb兔单抗检测内源性TIAR总蛋白。该抗体也较低程度的识别重组TIA1蛋白。通过人工合成人源TIAR蛋白羧基末端周围相应的多肽片段去免疫动物从而制备单克隆抗体。TIAR蛋白是一个RNA结合蛋白中RNA识别结构域(RRM)家族的成员(1,2)。在细胞内损伤的情况下，该蛋白的功能是作为一个翻译抑制性因子(3,4)。在细胞应激情况下，TIAR蛋白与eIF1、eIF3和40S核糖体亚基联系，并且形成非经典的翻译起始前复合物，该复合物不具备翻译活性 (3,4)。然后，TIAR蛋白能够与它的家族成员TIA1蛋白聚集，并且有助于蛋白质翻译失活的非经典起始前复合物进入被称为应激颗粒的离散细胞质颗粒中。TIAR蛋白的主要两个亚型是选择性mRNA剪切的产物 (5,6)。

1. Tian, Q. et al. (1991) Cell 67, 629-39.
2. Kawakami, A. et al. (1992) Proc Natl Acad Sci USA 89, 8681-5.
3. Anderson, P. and Kedersha, N. (2002) Cell Stress Chaperones 7, 213-21.
4. Anderson, P. and Kedersha, N. (2002) J Cell Sci 115, 3227-34.
5. Kawakami, A. et al. (1994) J Immunol 152, 4937-45.
6. Beck, A.R. et al. (1996) Nucleic Acids Res 24, 3829-35.

原厂资料：

Specificity / Sensitivity

TIAR (D32D3) XP® Rabbit mAb recognizes endogenous levels of total TIAR protein. This antibody also weakly recognizes recombinant TIA1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TIAR protein.

Background

TIAR is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins (1,2). It functions as a translational repressor under conditions of cellular damage (3,4). In response to cellular stress, TIAR associates with eIF1, eIF3, and the 40S ribosomal subunit and forms noncanonical preinitiation complexes that are translationally inactive (3,4). TIAR then aggregates with its family member TIA1 and facilitates the accumulation of the translationally inactive preinitiation complexes into discrete cytoplasmic foci called stress granules. The two major isoforms of TIAR are the products of alternative mRNA splicing (5,6).

1. Tian, Q. et al. (1991) Cell 67, 629-39.
2. Kawakami, A. et al. (1992) Proc Natl Acad Sci USA 89, 8681-5.
3. Anderson, P. and Kedersha, N. (2002) Cell Stress Chaperones 7, 213-21.
4. Anderson, P. and Kedersha, N. (2002) J Cell Sci 115, 3227-34.
5. Kawakami, A. et al. (1994) J Immunol 152, 4937-45.
6. Beck, A.R. et al. (1996) Nucleic Acids Res 24, 3829-35.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody