KIF3A (D7G3) Rabbit mAb

• 产品编号：CST-8507S      品牌：CST       原厂货号：8507S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

KIF3A (D7G3)Rabbit mAb可以识别内源性总的KIF3A 蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人KIF3A蛋白羧基端附近氨基酸一致。驱动蛋白超家族（KIFs）是一种分子马达驱动膜结合细胞器和其他大分子（例如蛋白，核酸）的方向性的，微管依赖的细胞内转运.KIFs的细胞内转运功能对于多种细胞功能是根本性的，包括有丝分裂和减数分裂，运动/迁移，激素和神经递质的释放，以及分化（1-4）。KIF-介导的胞内转运被破坏后会与一系列的病理状态相关，从肿瘤发生到高阶脑损伤，例如学习和记忆（4-6）。驱动蛋白超家族蛋白3A（KIF3A）是驱动蛋白-2复合物的重要组分（7）。KIF3A和它的横向同源物KIF3B结合形成异二聚体马达蛋白，具有ATP依赖的，plus-end-directed微管滑动作用（8）。该异二聚体的尾部与驱动蛋白-相关蛋白3（KAP3）集合，协助KIF3A/3B马达蛋白与它的cargo结合（7,8）。最近在多个模式生物中的研究证明驱动蛋白家族蛋白，包括KIF3A，在纤毛的形成和功能上具有重要作用（9）。特别地是，KIF3A介导了纤毛依赖的蛋白-蛋白相互作用发挥了转导经典Hedgehog信号通路（10）。

1. Hirokawa, N. et al. (2009) Nat Rev Mol Cell Biol 10, 682-96.
2. Yu, Y. and Feng, Y.M. (2010) Cancer 116, 5150-60.
3. Park, J.J. et al. (2008) Mol Endocrinol 22, 989-1005.
4. Hirokawa, N. et al. (2010) Neuron 68, 610-38.
5. Yoshimura, Y. et al. (2010) Mol Cell Biol 30, 2206-19.
6. Hirokawa, N. and Noda, Y. (2008) Physiol Rev 88, 1089-118.
7. Haraguchi, K. et al. (2006) J Biol Chem 281, 4094-9.
8. Yamazaki, H. et al. (1995) J Cell Biol 130, 1387-99.
9. Zhao, C. et al. (2012) Proc Natl Acad Sci U S A 109, 2388-93.
10. Humke, E.W. et al. (2010) Genes Dev 24, 670-82.

原厂资料：

Specificity / Sensitivity

KIF3A (D7G3) Rabbit mAb recognizes endogenous levels of total KIF3A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human KIF3A protein.

Background

Kinesin superfamily proteins (KIFs) are molecular motors that drive directional, microtubule-dependent intracellular transport of membrane-bound organelles and other macromolecules (e.g. proteins, nucleic acids). The intracellular transport functions of KIFs are fundamentally important for a variety of cellular functions, including mitotic and meiotic division, motility/migration, hormone and neurotransmitter release, and differentiation (1-4). Disruptions to KIF-mediated intracellular transport have been linked with a variety of pathologies, ranging from tumorigenesis to defects in higher order brain function, such as learning and memory (4-6).Kinesin superfamily protein 3A (KIF3A) is a central component of the kinesin-2 protein complex (7). KIF3A and its paralog KIF3B bind to form a heterodimeric motor protein with ATP-dependent, plus-end-directed microtubule sliding activity (8). The tail domain of this heterodimer binds to kinesin-associated protein 3 (KAP3), which facilitates binding of the KIF3A/3B motor protein to its cargo (7,8). Recent studies in a variety of model organisms have demonstrated a critical role for kinesin-family proteins, including KIF3A, in the formation and function of cilia (9). Notably, KIF3A was shown to mediate cilia-dependent protein-protein interactions that function to transduce canonical Hedgehog signaling (10).

1. Hirokawa, N. et al. (2009) Nat Rev Mol Cell Biol 10, 682-96.
2. Yu, Y. and Feng, Y.M. (2010) Cancer 116, 5150-60.
3. Park, J.J. et al. (2008) Mol Endocrinol 22, 989-1005.
4. Hirokawa, N. et al. (2010) Neuron 68, 610-38.
5. Yoshimura, Y. et al. (2010) Mol Cell Biol 30, 2206-19.
6. Hirokawa, N. and Noda, Y. (2008) Physiol Rev 88, 1089-118.
7. Haraguchi, K. et al. (2006) J Biol Chem 281, 4094-9.
8. Yamazaki, H. et al. (1995) J Cell Biol 130, 1387-99.
9. Zhao, C. et al. (2012) Proc Natl Acad Sci U S A 109, 2388-93.
10. Humke, E.W. et al. (2010) Genes Dev 24, 670-82.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.