USP10 (D7A5) Rabbit mAb

• 产品编号：CST-8501P      品牌：Cell Signaling Technology       原厂货号：8501P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

USP10 (D7A5) Rabbit mAb兔单抗识别内源性的USP10蛋白。该单克隆抗体是经合成与人USP10蛋白氨基末端邻近氨基酸残基序列一致的肽段，免疫动物产生。泛素化作用酶（UBEs）催化蛋白的泛素化，与去泛素化作用酶(DUB)的过程可逆(1,2)。5种DUB亚家族已经被确定，包括USP, UCH, OTU, MJD和JAMM酶。USP10具有与代表去泛素酶的USP家族的半胱氨酸和组氨酸盒子相匹配的氨基酸序列。在翻译后水平，USP10通过蛋白-蛋白相互作用和磷酸化被调节。确实，USP10与Ras-GAP SH3结构域结合蛋白(G3BP)的相互作用，可以抑制其催化泛素链分解的活性(3)。此外，ATM-介导的USP10的Thr42 和Ser337的磷酸化，可以促进USP10的稳定性和从胞质到核的重新分配，并在p53去泛素化、定位以及对具有遗传毒性压力应答的活性中发挥功效(4)。最近证实，USP10与USP13 和Vps34复合物具有相同的作用。USP10和USP13一起，呈现出对Vps34复合物的去泛素作用，进而调节III 类PI3K的水平。Beclin-1，这些复合物的其它组分，可以调节USP13的稳定性，能够去泛素化并稳定USP10的水平。因此，Beclin-1可以通过控制USP10的DUB活性间接地调节p53的稳定性(5)。USP10也在内涵室小体中起作用，在该小体中呈现出CFTR去泛素化作用，从而加强它的胞吞循环和细胞表面表达(6,7)。

1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
3. Soncini, C. et al. (2001) Oncogene 20, 3869-79.
4. Yuan, J. et al. (2010) Cell 140, 384-96.
5. Liu, J. et al. (2011) Cell 147, 223-34.
6. Bomberger, J.M. et al. (2009) J Biol Chem 284, 18778-89.
7. Bomberger, J.M. et al. (2011) Channels (Austin) 4, 150-4.

原厂资料：

Specificity / Sensitivity

USP10 (D7A5) Rabbit mAb recognizes endogenous levels of total USP10 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human USP10 protein.

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. USP10 possesses amino acid sequences that match the consensus cysteine and histidine boxes representative of the USP family of deubiquitinating enzymes. At the posttranslational level, USP10 appears to be regulated through both protein-protein interactions and phosphorylation. Indeed, interaction of USP10 with Ras-GAP SH3 domain binding protein (G3BP) has been found to inhibit its ability to catalyze the disassembly of ubiquitin chains (3). Furthermore, ATM-mediated phosphorylation of USP10 at Thr42 and Ser337 was shown to promote USP10 stabilization and redistribution from the cytoplasm to the nucleus, where it functions in p53 deubiquitination, stabilization, and activation in response to genotoxic stress (4). Recently, it was shown that USP10 works in concert with USP13 and Vps34 complexes. USP10, along with USP13, appears to deubiquitinate Vps34 complexes to regulate the levels of this class III PI3K. Beclin-1, another component of these complexes, functions to regulate the stability of USP13, which can deubiquitinate and stabilize the levels of USP10. Therefore, Beclin-1, can indirectly regulate p53 stability by controlling the DUB activity of USP10 (5). USP10 also functions in the endosomal compartment, where it has been shown to deubiquitinate CFTR in order to enhance its endocytic recycling and cell surface expression (6,7).

1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
3. Soncini, C. et al. (2001) Oncogene 20, 3869-79.
4. Yuan, J. et al. (2010) Cell 140, 384-96.
5. Liu, J. et al. (2011) Cell 147, 223-34.
6. Bomberger, J.M. et al. (2009) J Biol Chem 284, 18778-89.
7. Bomberger, J.M. et al. (2011) Channels (Austin) 4, 150-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.