Sestrin-2 (D1B6) Rabbit mAb

• 产品编号：CST-8487S      品牌：CST       原厂货号：8487S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Sestrin-2 (D1B6) Rabbit mAb能够检测过表达的sestrin-2总蛋白，及表达水平较高的内源性sestrin-2蛋白。该单克隆抗体是由合成的人源的针对sestrin-2蛋白的氨基末端肽段免疫动物生产的。Sestrin蛋白家族，包括sestrin-1（PA26）、sestrin-2（Hi95）和sestrin-3，在调节细胞的氧化应激反应中起着重要的作用。 sestrin-1和-2，两个都是p53基因的转录靶点，被显示能够激活针对TSC2的AMPK活性，最终导致mTOR抑制（1）。因此，sestrin-1和-2连接p53信号至细胞生长调节（2）。也已证明sestrins能够充当细胞的过氧化氢浓度的调节器。过氧化是一个重要的信号传导分子，具有高度遗传毒性，因此，必须严格进行调控（3）。2-半胱氨酸过氧化物酶是能够保持较低的细胞过氧化氢浓度的代谢酶，但是在迅速地减少并通过sestrins返回到它们的激活状态之前(5)，该酶被迅速增加的过氧化氢浓度氧化到非活化状态(4)。这允许过氧化氢信号暂时规避2-半胱氨酸过氧化物酶的保护性活性，同时最大限度地减少氧化损伤。

1. Budanov, A.V. and Karin, M. (2008) Cell 134, 451-60.
2. Wullschleger, S. et al. (2006) Cell 124, 471-84.
3. Veal, E.A. et al. (2007) Mol Cell 26, 1-14.
4. Jönsson, T.J. and Lowther, W.T. (2007) Subcell Biochem 44, 115-41.
5. Budanov, A.V. et al. (2004) Science 304, 596-600.

原厂资料：

Specificity / Sensitivity

Sestrin-2 (D1B6) Rabbit mAb recognizes overexpressed levels of total sestrin-2 protein, as well as endogenous levels of sestrin-2 protein when expression is high.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human sestrin-2 protein.

Background

The Sestrin family of proteins, which comprises sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, plays an important role in regulating the cellular response to oxidative stress. Sestrin-1 and -2, both transcriptional targets of p53, have been shown to activate AMPK activity toward TSC2, ultimately resulting in the inhibition of mTOR (1). Thus, sestrin-1 and -2 link p53 signaling to the regulation of cellular growth (2). Sestrins have also been shown to function as modulators of cellular hydrogen peroxide concentration. Hydrogen peroxide is an important signaling molecule that is highly genotoxic and must therefore be tightly regulated (3). 2-Cys peroxiredoxins are metabolizing enzymes that maintain low cellular concentrations of hydrogen peroxide, but are hyperoxidized to an inactive state by a rapid increase in concentration (4) before quickly being reduced back to their active state by sestrins (5). This allows a hydrogen peroxide signal to temporarily circumvent the protective activity of 2-Cys peroxiredoxins, while minimizing oxidative damage.

1. Budanov, A.V. and Karin, M. (2008) Cell 134, 451-60.
2. Wullschleger, S. et al. (2006) Cell 124, 471-84.
3. Veal, E.A. et al. (2007) Mol Cell 26, 1-14.
4. Jönsson, T.J. and Lowther, W.T. (2007) Subcell Biochem 44, 115-41.
5. Budanov, A.V. et al. (2004) Science 304, 596-600.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody