IRF-1 (D5E4) XP? Rabbit mAb

• 产品编号：CST-8478P      品牌：Cell Signaling Technology       原厂货号：8478P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IRF-1 (D5E4) XP® Rabbit mAb能够检测内源IRF-1蛋白总体水平。此单克隆抗体是通过合成人源对应的IRF-1 Pro261位点周围的肽段来免疫动物而获得。干扰素调控因子(IRFs) 包含一个家族的转录调控因子，这个家族主要是在 Jak/Stat信号通路中起作用，在受到病毒的感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌、自体免疫、淋巴细胞发育、细胞生长和易感性的转变等过程中发挥了重要的作用。IRF家族包含9个成员: IRF-1、IRF-2、ISGF3γ/p48、IRF-3、 IRF-4 (Pip/LSIRF/ICSAT)、 IRF-5、 IRF-6、 IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都具有同源性。IRF家族成员通过与具有相似DNA-结合区域（例如IFN刺激的应答原件(ISRE)、IFN consensus序列 (ICS) 和IFN 调控元件(IRF-E)） 的蛋白相互作用而调控转录(2)。IRF-1转录因子最初被鉴定为病毒诱导 IFN-β基因的增强子样元件的调控分子(3)。IRF-1广泛表达，并被病毒感染和IFN或其它细胞因子的刺激而上调表达。IRF-1通过酪蛋白激酶II (CKII)将两个丝氨酸簇的位点磷酸化, 一个位于DNA-结合区域(氨基酸138-150)，另一个在转录激活区域(氨基酸219-231) (4)。突变分析后面一个磷酸化位点暗示了这些磷酸化位点能帮助调控IRF-1活性。酪氨酸磷酸化在 IFN-γ介导的骨髓细胞系分化过程中呈现了重要的作用(5)。C-末端SUMO化修饰IRF-1 通过抑制其转录活性而抑制肿瘤细胞的凋亡(6)。

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Fujita, T. et al. (1988) EMBO J 7, 3397-405.
4. Lin, R. and Hiscott, J. (1999) Mol Cell Biochem 191, 169-80.
5. Kautz, B. et al. (2001) J Biol Chem 276, 37868-78.
6. Park, J. et al. (2007) Proc Natl Acad Sci USA 104, 17028-33.

原厂资料：

Specificity / Sensitivity

IRF-1 (D5E4) XP® Rabbit mAb recognizes endogenous levels of total IRF-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro261 of human IRF-1 protein.

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).The IRF-1 transcription factor was originally identified as a regulator of virus-inducible enhancer-like elements of the IFN-β gene (3). IRF-1 is widely expressed and upregulated by viral infection or stimulation with IFN or other cytokines. IRF-1 is serine-phosphorylated by casein kinase II (CKII) at two clustered sites, one in the DNA-binding domain (amino acids 138-150) and another in the transactivation domain (amino acids 219-231) (4). Mutation analysis of the latter site suggests that these phosphorylation sites help regulate IRF-1 activity. Tyrosine phosphorylation has also been shown to be important in IFN-γ-mediated differentiation of myeloid cell lines (5). C-terminal SUMOylated IRF-1 inhibits apoptosis in tumor cells by repression of its transcriptional activity (6).

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Fujita, T. et al. (1988) EMBO J 7, 3397-405.
4. Lin, R. and Hiscott, J. (1999) Mol Cell Biochem 191, 169-80.
5. Kautz, B. et al. (2001) J Biol Chem 276, 37868-78.
6. Park, J. et al. (2007) Proc Natl Acad Sci USA 104, 17028-33.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.