Rubicon (D9F7) Rabbit mAb

• 产品编号：CST-8465S      品牌：CST       原厂货号：8465S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rubicon (D9F7) Rabbit mAb 兔单抗能够识别内源性的Rubicon总蛋白。在55kDa处检测到一条来源不明的条带。该单克隆抗体是用合成的人Rubicon蛋白 Leu210 位点周围的残基相应的肽段免疫动物制备而得。自噬是一个自噬溶酶体降解其所包裹的细胞质内容物的分解代谢过程。通常情况下，自噬在营养匮乏的时候被激活，但它还与许多生理过程相关，包括生长、分化、神经退行性疾病、感染和癌症。自噬的分子机制主要在酵母中发现的，并被称之为自噬相关基因。这些蛋白与自噬体形成有关，自噬体就是被送往溶酶体进行降解的胞浆空泡。III型磷酸肌醇3激酶（PI3K）Vps34调节空泡的运输和自噬（4,5）。许多蛋白包括p105/Vsp15、Beclin-1、UVRAG、Atg14和Rubicon与Vsp34的功能相关（6-12)。tg14L和Rubicon能够与Beclin-1结合，并且参与具有相对功能的独特复合物的形成（9-12）。 Rubicon主要集中于内涵体和溶酶体，能够抑制Vps34脂质激酶活性；Rubicon的下调可以增强自噬和内吞作用（11,12）。相反，Atg14L集中于自噬体、隔离膜和内质网，能够增强Vps34活性；Atg14L的下调可以抑制饥饿诱导的自噬。

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
4. Corvera, S. (2001) Traffic 2, 859-66.
5. Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.
6. Stack, J.H. et al. (1995) J Cell Biol 129, 321-34.
7. Zeng, X. et al. (2006) J Cell Sci 119, 259-70.
8. Liang, C. et al. (2006) Nat Cell Biol 8, 688-99.
9. Itakura, E. et al. (2008) Mol Biol Cell 19, 5360-72.
10. Sun, Q. et al. (2008) Proc Natl Acad Sci USA 105, 19211-6.
11. Zhong, Y. et al. (2009) Nat Cell Biol 11, 468-76.
12. Matsunaga, K. et al. (2009) Nat Cell Biol 11, 385-96.

原厂资料：

Specificity / Sensitivity

Rubicon (D9F7) Rabbit mAb recognizes endogenous levels of total Rubicon protein. A band of unknown origin is detected at 55 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu210 of human Rubicon protein.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. These proteins are involved in the formation of autophagosomes, which are cytoplasmic vacuoles that are delivered to lysosomes for degradation. The class III type phosphoinositide 3-kinase (PI3K) Vps34 regulates vacuolar trafficking and autophagy (4,5). Multiple proteins associate with Vsp34, including p105/Vsp15, Beclin-1, UVRAG, Atg14, and Rubicon (6-12). Atg14L and Rubicon were identified based on their ability to bind to Beclin-1 and participate in unique complexes with opposing functions (9-12). Rubicon, which localizes to the endosome and lysosome, inhibits Vps34 lipid kinase activity; knockdown of Rubicon enhances autophagy and endocytic trafficking (11,12). In contrast, Atg14L localizes to autophagosomes, isolation membranes, and ER and can enhance Vps34 activity. Knockdown of Atg14L inhibits starvation-induced autophagy (11,12).

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
4. Corvera, S. (2001) Traffic 2, 859-66.
5. Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.
6. Stack, J.H. et al. (1995) J Cell Biol 129, 321-34.
7. Zeng, X. et al. (2006) J Cell Sci 119, 259-70.
8. Liang, C. et al. (2006) Nat Cell Biol 8, 688-99.
9. Itakura, E. et al. (2008) Mol Biol Cell 19, 5360-72.
10. Sun, Q. et al. (2008) Proc Natl Acad Sci USA 105, 19211-6.
11. Zhong, Y. et al. (2009) Nat Cell Biol 11, 468-76.
12. Matsunaga, K. et al. (2009) Nat Cell Biol 11, 385-96.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.