R-Ras Antibody

• 产品编号：CST-8446S      品牌：CST       原厂货号：8446S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

R-Ras Antibody检测内源性R-Ras总蛋白。通过人工合成人源R-Ras蛋白氨基端相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。R-Ras蛋白是一个小GTPase家族成员，该蛋白与H-、K-和N-Ras有同源性，但是不能激活MAP激酶通路和较弱的致癌作用(1)。相反，R-Ras蛋白从事整合素的激活(2)。R-Ras蛋白的效应分子环和羧基端脯氨酸富足区和异戊烯位点对整合素的激活是至关重要的(3,4)。通过EphB2受体酪氨酸激酶和Src使R-Ras蛋白Tyr66位点的磷酸化抑制整合素的活性(5,6)。

1. Self, A. J. et al. (2001) J. Cell Sci. 114, 1357-1366.
2. Zhang, Z. et al. (1996) Cell 85, 61-69.
3. Oertli, B. et al. (2000) Oncogene 19, 4961-4969.
4. Wang, B. et al. (2000) J Biol Chem 275, 5222-7.
5. Zou, J.X. et al. (1999) Proc Natl Acad Sci U S A 96, 13813-8.
6. Zou, J.X. et al. (2002) J Biol Chem 277, 1824-7.

原厂资料：

Specificity / Sensitivity

R-Ras Antibody recognizes endogenous levels of total R-Ras protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human R-Ras protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

R-Ras, a member of the small GTPase family, is homologous to H-, K- and N-Ras, but does not activate MAP kinase pathways and is only weakly oncogenic (1). Instead, R-Ras is engaged in integrin activation (2). The effector loop and the carboxy-terminal proline-rich and prenylation sites of R-Ras are critical for integrin activation (3,4). Phosphorylation by EphB2 receptor tyrosine kinase and Src at Tyr66 of R-Ras suppresses integrin activity (5,6).

1. Self, A. J. et al. (2001) J. Cell Sci. 114, 1357-1366.
2. Zhang, Z. et al. (1996) Cell 85, 61-69.
3. Oertli, B. et al. (2000) Oncogene 19, 4961-4969.
4. Wang, B. et al. (2000) J Biol Chem 275, 5222-7.
5. Zou, J.X. et al. (1999) Proc Natl Acad Sci U S A 96, 13813-8.
6. Zou, J.X. et al. (2002) J Biol Chem 277, 1824-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.