hnRNP A1 (D21H11) Rabbit mAb

• 产品编号：CST-8443S      品牌：CST       原厂货号：8443S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

hnRNP A1 (D21H11) Rabbit mAb兔单抗检测内源性hnRNP A1总蛋白水平。这个抗体不能与hnRNP家族蛋白发生交叉反应。通过人工合成人源hnRNP A1蛋白Gly209位点周围序列相应的多肽片段去免疫动物从而制备出单克隆抗体。Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1)是RNA结合蛋白相关的hnRNP A/B家族成员之一。该类蛋白能够结合pre-mRNA，同时参与RNA的加工、代谢和核内pre-mRNA转录本的转运(1)。hnRNP A1蛋白调控c-Src和c-H-Ras(2,3)的可变剪接，同时影响成纤维细胞生长因子2 mRNA的翻译起始(4)。在许多癌症细胞中hnRNP A1蛋白表达水平上升；并且hnRNP A1基因沉默可导致许多癌细胞的凋亡(5)。虽然hnRNP A1 主要是在细胞核内，但hnRNP A1不断的从细胞核转运到细胞质中，并在这里与mRNA解离并快速被重新转运至核内(6,7)。 hnRNP A1蛋白能结合HIV-1的顺式作用反应序列(CRS)从而影响HIV-1的生成(8,9)。 HIV-1能提高hnRNP A1的表达，并且促进hnRNP A1重新定位到细胞质(10)。

1. Myer, V.E. and Steitz, J.A. (1995) RNA 1, 171-82.
2. Rooke, N. et al. (2003) Mol Cell Biol 23, 1874-84.
3. Guil, S. et al. (2003) Mol Cell Biol 23, 2927-41.
4. Bonnal, S. et al. (2005) J Biol Chem 280, 4144-53.
5. Patry, C. et al. (2003) Cancer Res 63, 7679-88.
6. Piñol-Roma, S. and Dreyfuss, G. (1992) Nature 355, 730-2.
7. Siomi, M.C. et al. (1997) J Cell Biol 138, 1181-92.
8. Black, A.C. et al. (1996) Virus Genes 12, 275-85.
9. Hadian, K. et al. (2009) J Biol Chem 284, 33384-91.
10. Monette, A. et al. (2009) J Biol Chem 284, 31350-62.

原厂资料：

Specificity / Sensitivity

hnRNP A1 (D21H11) Rabbit mAb recognizes endogenous levels of total hnRNP A1 protein. This antibody does not cross react with other hnRNP family member proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly209 of human hnRNP A1 protein.

Background

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). hnRNP A1 regulates the alternative splicing of c-Src and c-H-Ras (2,3) and modifies initiation of translation of the fibroblast growth factor 2 mRNA (4). hnRNP A1 expression level is elevated in many cancers; knockdown of hnRNP A1 leads to apoptosis in various cancer cells (5). Although predominantly nuclear, hnRNP A1 is continually transported from the nucleus to the cytoplasm where it disassociates from mRNA and is rapidly re-imported into the nucleus (6,7). hnRNP A1 binds to cis-acting repressive sequences (CRS) of HIV-1 to influence HIV-1 production (8,9). HIV-1 enhances hnRNP A1 expression and promotes the relocalization of hnRNP A1 to the cytoplasm (10).

1. Myer, V.E. and Steitz, J.A. (1995) RNA 1, 171-82.
2. Rooke, N. et al. (2003) Mol Cell Biol 23, 1874-84.
3. Guil, S. et al. (2003) Mol Cell Biol 23, 2927-41.
4. Bonnal, S. et al. (2005) J Biol Chem 280, 4144-53.
5. Patry, C. et al. (2003) Cancer Res 63, 7679-88.
6. Piñol-Roma, S. and Dreyfuss, G. (1992) Nature 355, 730-2.
7. Siomi, M.C. et al. (1997) J Cell Biol 138, 1181-92.
8. Black, A.C. et al. (1996) Virus Genes 12, 275-85.
9. Hadian, K. et al. (2009) J Biol Chem 284, 33384-91.
10. Monette, A. et al. (2009) J Biol Chem 284, 31350-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.