Phospho-Rad18 (Ser403) Antibody

• 产品编号：CST-8393S      品牌：CST       原厂货号：8393S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Rad18 (Ser403) Antibody能够检测内源性丝氨酸（403位）磷酸化的Rad18蛋白。在一些细胞系中，该抗体与一个大于200kDa的不明来源蛋白发生交叉反应。该多克隆抗体是由合成的人源的针对Rad18蛋白丝氨酸（403位）的肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。DNA损伤，如果不进行修复，能够导致基因组不稳定和肿瘤发生。真核细胞采用多个(有时会有重叠)信号通路应答引起各种类型DNA损伤的因素。DNA修复中的下游分子集中于DNA损伤位点，引起细胞周期捕获和修复或凋亡(1)。Rad18是一种可被招募至DNA损伤位点的E3泛素连接酶。连同E2泛素连接酶rad16，rad18负责DNA损伤蛋白(包括复制夹蛋白PCNA和Fanconi贫血核心蛋白FANCD2)的单泛素化。这些蛋白的单泛素化给下游的效应器分子发送信号并且通过跨损伤合成途径进行损伤后修复或者通过同源重组修复DNA双链断裂(2-4)。磷酸蛋白质组学研究表明rad18的第403位丝氨酸可以被ATM/ATR磷酸化以响应DNA损伤诱导物(5,6)。

1. Helleday, T. et al. (2008) Nat Rev Cancer 8, 193-204.
2. Huang, J. et al. (2009) Nat Cell Biol 11, 592-603.
3. Song, I.Y. et al. (2010) J Biol Chem 285, 31525-36.
4. Ting, L. et al. (2010) DNA Repair (Amst) 9, 1241-8.
5. Mu, J.J. et al. (2007) J Biol Chem 282, 17330-4.
6. Matsuoka, S. et al. (2007) Science 316, 1160-6.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Phospho-Rad18 (Ser403) Antibody recognizes endogenous levels of Rad18 protein only when phosphorylated at Ser403. In some cell types, the antibody cross-reacts with a >200 kDa protein of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser403 of human Rad18 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

DNA damage, if not repaired, can lead to genome instability and tumorigenesis. Eukaryotic cells use multiple (sometimes overlapping) signaling pathways to respond to agents that cause various types of DNA lesions. Downstream molecules in DNA repair pathways converge on the sites of DNA damage, resulting in cell cycle arrest and repair or apoptosis (1). Rad18 is an E3 ubiquitin ligase recruited to sites of DNA damage. Along with the E2 ubiquitin ligase rad6, rad18 is responsible for monoubiquitination of DNA damage proteins including the replication clamp PCNA and the Fanconi anemia core protein FANCD2. Monoubiquitination of these proteins signals to downstream effector molecules and results in the repair of either post-replication repair lesions via the translesion synthesis (TLS) pathway or of DNA double strand breaks via homologous recombination (2-4). Phospho-proteomic studies indicate that serine 403 of rad18 may be phosphorylated by ATM/ATR in response to DNA damage-inducing agents (5,6). 

1. Helleday, T. et al. (2008) Nat Rev Cancer 8, 193-204.
2. Huang, J. et al. (2009) Nat Cell Biol 11, 592-603.
3. Song, I.Y. et al. (2010) J Biol Chem 285, 31525-36.
4. Ting, L. et al. (2010) DNA Repair (Amst) 9, 1241-8.
5. Mu, J.J. et al. (2007) J Biol Chem 282, 17330-4.
6. Matsuoka, S. et al. (2007) Science 316, 1160-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody