Glycolysis Antibody Sampler Kit

• 产品编号：CST-8337S      品牌：Cell Signaling Technology       原厂货号：8337S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

GAPDH (D16H11) XP® Rabbit mAb, Hexokinase I (C35C4) Rabbit mAb, Hexokinase II (C64G5) Rabbit mAb, LDHA (C4B5) Rabbit mAb, PKM2 (D78A4) XP® Rabbit mAb,和PFKP Antibody分别用于检测它们相对应的内源性总蛋白水平。Pyruvate Dehydrogenase (C54G1) Rabbit mAb 用于检测内源性丙酮酸脱氢酶α1亚 基总蛋白水平。PKM1/2 (C103A3) Rabbit mAb用于检测内源性PKM(包括M1和M2)总蛋白水平。单抗的制备：用与人GAPDH，已糖激酶I，已糖激酶II，LDHA, PKM2, 和丙酮酸脱氢酶序列相同的合成肽免疫动物制备而得。多抗通过用与人源PFKP蛋白N端序列相同的合成肽免疫动物制备而来。多抗的纯化采用蛋白A与肽段亲和层析相结合的方法。Glycolysis Antibody Sampler Kit为研究糖酵解过程中的一些酶的水平，提供了一套经济有效的解决方案。试剂盒中的每种一抗都至少可以满足四次Western blot实验。糖酵解指的是葡萄糖在一系列酶催化反应下转变成丙酮酸的过程。糖酵解的第一步是葡萄糖在已糖激酶催化下转变成6-磷酸葡萄糖。已糖激酶I, II, 和III都 与线粒体外膜相连，它们对维持肿瘤细胞高速的有氧糖酵解是必需的(Warburg效应)(1)。磷酸果糖激酶(PFK)负责将6-磷酸果糖进一步磷酸化。血小板样的磷 酸果糖激酶在多种细胞类型都有表达(2)。3-磷酸甘油醛脱氢酶(GAPDH)可以将3-磷酸甘油醛再次磷酸化(3)。丙酮酸激酶作为一种糖分解酶类，可以将磷酸烯 醇式丙酮酸转变成丙酮酸。在哺乳类中，M1(PKM1)型同工酶主要在成年人组织中表达。而M2(PKM2)型同工酶(是M1的可变剪切形式)主要在胚胎发育期表达(4) 。乳酸脱氢酶可以将丙酮酸和NADH催化成乳酸和NAD+，这是一个可逆反应(5)。当线粒体产ATP的供氧不足时，LDHA的表达就被诱导出来(6)。在存在NAD+的前 提下，丙酮酸脱氢酶复合体可以将丙酮酸和辅酶A催化成乙酰辅酶A及CO2。丙酮酸的氧化脱羧反应是糖酵解，柠檬酸循环和脂质代谢的一个重要连接(7)。

原厂资料：

Specificity / Sensitivity

GAPDH (D16H11) XP® Rabbit mAb, Hexokinase I (C35C4) Rabbit mAb, Hexokinase II (C64G5) Rabbit mAb, LDHA (C4B5) Rabbit mAb, PKM2 (D78A4) XP® Rabbit mAb, and PFKP Antibody all detect endogenous levels of their respective total proteins. Pyruvate Dehydrogenase (C54G1) Rabbit mAb detects endogenous levels of total pyruvate dehydrogenase α1 subunit. PKM1/2 (C103A3) Rabbit mAb detects endogenous levels of total PKM (including M1 and M2) protein.

Source / Purification

Monoclonal antibodies were produced by immunizing animals with a synthetic peptide corresponding to the respective sequences of human GAPDH, hexokinase I, hexokinase II, LDHA, PKM2, or pyruvate dehydrogenase. Polyclonal antibodies were produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PFKP protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Glycolysis Antibody Sampler Kit provides an economical means to investigate select enzymes involved in glycolysis. The kit contains enough primary antibody to perform four western blot experiments with each primary antibody.

Background

Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg effect) (1). Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate. Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (2). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate (3). Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (4). Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+ (5). LDHA expression is induced when the oxygen supply is too low for mitochondrial ATP production (6). The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. The reaction of oxidative decarboxylation of pyruvate serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism (7).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 μg/ml BSA and 50% glycerol. Store at –20°C.