ROCK2 Antibody

• 产品编号：CST-8236S      品牌：CST       原厂货号：8236S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ROCK2 Antibody检测内源性ROCK2总蛋白。通过人工合成人源ROCK2蛋白羧基末端相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。ROCK (Rho-associated kinase)是丝氨酸/苏氨酸激酶家族，该蛋白是GTPase Rho的重要下游靶分子，并且在Rho介导信号中起着重要作用。ROCK蛋白的两个亚型已经被鉴定（ROCK1和ROCK2）。ROCK蛋白是由N-端催化、卷曲螺旋（coiled coil）结构和C-端PH (pleckstrin homology)结构组成。ROCK蛋白的C-端负性调节它的激酶活性(1,2)。Caspase-3诱导的ROCK1的剪切和由granzyme B (grB)直接剪切ROCK2蛋白激活ROCK蛋白，并且导致肌球蛋白轻链 的磷酸化和肌球蛋白磷酸酶的抑制(3)。 该磷酸化过程可能说明一个机制那就是通过Rho可调节细胞分裂、细胞运动、凋亡期间细胞膜出泡和平滑肌收缩(4-6)。

1. Nakagawa, O. et al. (1996) FEBS Lett. 392, 189-193.
2. Lee, J.H. et al. (2004) J. Cell. Biol. 167, 327-337.
3. Sebbagh, M. et al. (2005) J. Exp. Med. 201, 465-471.
4. Amano, M. et al. (1996) J. Biol. Chem. 271, 20246-20249.
5. Kureishi, Y. et al. (1997) J. Biol. Chem. 272, 12257-12260.
6. Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

ROCK2 Antibody recognizes endogenous levels of total ROCK2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ROCK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of GTPase Rho and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified (ROCK1 and ROCK2). ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase (3). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (4-6).

1. Nakagawa, O. et al. (1996) FEBS Lett. 392, 189-193.
2. Lee, J.H. et al. (2004) J. Cell. Biol. 167, 327-337.
3. Sebbagh, M. et al. (2005) J. Exp. Med. 201, 465-471.
4. Amano, M. et al. (1996) J. Biol. Chem. 271, 20246-20249.
5. Kureishi, Y. et al. (1997) J. Biol. Chem. 272, 12257-12260.
6. Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.