AML1 Antibody (Mouse Preferred)

• 产品编号：CST-8229S      品牌：CST       原厂货号：8229S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AML1 antibody(优先用于小鼠)能够检测内源性AML1总蛋白水平。此抗体能够识别人源AML1，但是我们推荐用AML1 (D33G6) XP® Rabbit mAb #4336抗体检测人源的AML1蛋白。此多克隆抗体是通过合成人源对应的AML1 Val211位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。AML1 (也叫做Runx1, CBFA2 或 PEBP2αB)是转录因子核心结合因子(CBF)家族中的一员(1,2)。它对造血细胞系的正常发育是不可缺少的(3-5)。AML1与其伴侣蛋白CBFβ形成异二聚体DNA结合复合体，并通过结合启动子和增强子原件而调控细胞基因的表达。AML1 在造血相关癌症中通常发生染色体易位，易位的位置包括t(8;21) AML1-ETO、t(12;21) TEL-AML 和 t(8;21) AML-M2 (6)。磷酸化AML1的几个潜在丝氨酸和苏氨酸位点包括Ser249, 被认为是通过Erk依赖的方式而发生修饰 (7,8)。

1. Wang, S. et al. (1993) Mol Cell Biol 13, 3324-39.
2. Ogawa, E. et al. (1993) Proc Natl Acad Sci U S A 90, 6859-63.
3. Okuda, T. et al. (1996) Cell 84, 321-30.
4. Wang, Q. et al. (1996) Proc Natl Acad Sci U S A 93, 3444-9.
5. North, T.E. et al. (2004) Stem Cells 22, 158-68.
6. Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-87.
7. Tanaka, T. et al. (1996) Mol Cell Biol 16, 3967-79.
8. Zhang, Y. et al. (2004) J Biol Chem 279, 53116-25.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey

Specificity / Sensitivity

AML1 Antibody (Mouse Preferred) detects endogenous levels of total AML1 protein. This antibody will recognize human AML1, however, we recommend AML1 (D33G6) XP® Rabbit mAb #4336 for detection of human AML1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val211 of human AML1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).

1. Wang, S. et al. (1993) Mol Cell Biol 13, 3324-39.
2. Ogawa, E. et al. (1993) Proc Natl Acad Sci U S A 90, 6859-63.
3. Okuda, T. et al. (1996) Cell 84, 321-30.
4. Wang, Q. et al. (1996) Proc Natl Acad Sci U S A 93, 3444-9.
5. North, T.E. et al. (2004) Stem Cells 22, 158-68.
6. Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-87.
7. Tanaka, T. et al. (1996) Mol Cell Biol 16, 3967-79.
8. Zhang, Y. et al. (2004) J Biol Chem 279, 53116-25.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.