ZO-1 (D7D12) Rabbit mAb

• 产品编号：CST-8193S      品牌：CST       原厂货号：8193S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ZO-1 (D7D12) Rabbit mAb兔单抗检测内源性ZO-1总蛋白。通过人工合成人源ZO-1蛋白羧基端相应的多肽片段去免疫动物从而制备出单克隆抗体。紧密连接或zona occludens蛋白形成一个连续的屏障去阻碍液体穿过上皮细胞和内皮。它们的功能是调节细胞旁通透性和细胞极性的维持，这在顶端和基底的细胞表面之间阻碍了跨膜蛋白的运输(1)。Zona occludens蛋白ZO-1、-2和-3(又称TJP1、2和3)外围膜接头蛋白，它能联系紧密跨膜蛋白例如occludin和claudin蛋白到肌动蛋白细胞骨架(2)。ZO-1和-2 蛋白对于紧密连接的形成和功能是需要的(3,4)。在低汇合增殖的细胞中，ZO-1和ZO-2蛋白已经显示共定位于细胞核，并且在转录调节起到重要角色，这可能通过有助于转录因子的细胞核进入和输出(5-7)。ZO-2基因是来自两个启动子促进转录，这就产生ZO-2A和ZO-2C亚型。ZO-2C缺少一个23氨基酸氨基端序列，而该序列在其它 ZO-2亚型中被发现。当两个亚型都出现广泛表达时，ZO-2基因的不正常调节可能是与乳腺浸润性导管癌的发展有关联(8)。

1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
2. Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11.
3. Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47.
4. Umeda, K. et al. (2006) Cell 126, 741-54.
5. Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66.
6. Traweger, A. et al. (2003) J Biol Chem 278, 2692-700.
7. Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36.
8. Chlenski, A. et al. (2000) Biochim Biophys Acta 1493, 319-24.

原厂资料：

Specificity / Sensitivity

ZO-1 (D7D12) Rabbit mAb recognizes endogenous levels of total ZO-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ZO-1 protein.

Background

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and -2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).

1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
2. Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11.
3. Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47.
4. Umeda, K. et al. (2006) Cell 126, 741-54.
5. Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66.
6. Traweger, A. et al. (2003) J Biol Chem 278, 2692-700.
7. Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36.
8. Chlenski, A. et al. (2000) Biochim Biophys Acta 1493, 319-24.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody