FoxA2/HNF3β (D56D6) XP? Rabbit mAb

• 产品编号：CST-8186P      品牌：Cell Signaling Technology       原厂货号：8186P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FoxA2/HNF3β (D56D6) XP® Rabbit mAb兔单抗用于检测内源性FoxA2/HNF3β总蛋白水平。该单抗通过用与人源FoxA2/HNF3β蛋白Gly138位点周围残基序列相同的合成肽免疫动物制备而得。Forkhead盒式蛋白A2(FoxA2，同样也称作肝脏核转录因子3β即HNF3β)是一个在肝脏细胞中发挥功能的重要转录因子(1)。在饥饿状态下，肝脏的糖异生作用的相关基因的激活必须FoxA2/HNF3β的参与(1)。和辅转录因子 PGC-1β一起，FoxA2/HNF3β能够激活脂肪酸β氧化过程中的基因，因此它也可以增加脂肪酸代谢(2)。FoxA2/HNF3β和PGC-1β一起可以激活微小体甘油三酯转运蛋白(MTP)的表达，促进VLDL的分泌(2)。除了在代谢综合症中发挥的作用外，FoxA2/HNF3β对小鼠胚胎发育期内胚层和中线的发育也是必需的(3-5)。

1. Zhang, L. et al. (2005) Cell Metab 2, 141-8.
2. Wolfrum, C. and Stoffel, M. (2006) Cell Metab 3, 99-110.
3. Levinson-Dushnik, M. and Benvenisty, N. (1997) Mol Cell Biol 17, 3817-22.
4. Weinstein, D.C. et al. (1994) Cell 78, 575-88.
5. Ang, S.L. and Rossant, J. (1994) Cell 78, 561-74.

原厂资料：

Specificity / Sensitivity

FoxA2/HNF3β (D56D6) XP® Rabbit mAb recognizes endogenous levels of total FoxA2/HNF3β protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly138 of human FoxA2/HNF3β protein.

Background

Forkhead box protein A2 (FoxA2, also known as hepatocyte nuclear factor 3β or HNF3β) is a transcription factor that plays an important role in hepatocyte function (1). FoxA2/HNF3β is required for the activation of hepatic gluconeogenic gene expression during fasting (1). Together with the PGC-1β coactivator, FoxA2/HNF3β stimulates the expression of genes involved in fatty acid β-oxidation and therefore increases fatty acid metabolism (2). FoxA2/HNF3β, along with PGC-1β, also activates the expression of microsomal triacylglycerol transfer protein (MTP) and promotes VLDL secretion (2). In addition to its roles in metabolic syndromes, FoxA2/HNF3β is essential for development of the endoderm and midline structures in mouse embryos (3-5).

1. Zhang, L. et al. (2005) Cell Metab 2, 141-8.
2. Wolfrum, C. and Stoffel, M. (2006) Cell Metab 3, 99-110.
3. Levinson-Dushnik, M. and Benvenisty, N. (1997) Mol Cell Biol 17, 3817-22.
4. Weinstein, D.C. et al. (1994) Cell 78, 575-88.
5. Ang, S.L. and Rossant, J. (1994) Cell 78, 561-74.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.