C/EBPα (D56F10) XP? Rabbit mAb

• 产品编号：CST-8178S      品牌：CST       原厂货号：8178S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

C/EBPα (D56F10) XP?兔单克隆抗体识别内源性的C/EBPα总蛋白水平。该单克隆抗体用与人类C/EBPα蛋白中Ala176位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。CCAAT/增强子结合蛋白（C/EBP）是一个转录因子家族，对细胞分化、终端功能和炎症反应十分关键（1）。目前已确定该家族的6个成员（C/EBPα, β, δ, γ, ε, ζ），它们分布在不同组织（1）。由不同的启动子开始转录，可以得到两种不同的C/EBPα（p42和p30），两者均是强转录激活因子（2）。有报道发现胰岛素和胰岛素样生长因子I刺激C/EBPα的去磷酸化，在胰岛素诱导的GLUT4转录抑制中扮演重要角色（3）。C/EBPα被GSK-3在Thr222, Thr226, 和Ser230的磷酸化似乎在脂肪生成中是必需的（4）。

1. Lekstrom-Hims, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
2. Lin, F. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 9606-9610.
3. Hemati, N. et al. (1997) J. Biol. Chem. 272, 25913-25919.
4. Ross, S.E. et al. (1999) Mol. Cell. Biol. 19, 8433-8441.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

C/EBPα (D56F10) XP® Rabbit mAb recognizes endogenous levels of total C/EBPα protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala176 of human C/EBPα protein.

Background

CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function, and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε, and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-I stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin-induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226, and Ser230 by GSK-3 seems to be required for adipogenesis (4).

1. Lekstrom-Hims, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
2. Lin, F. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 9606-9610.
3. Hemati, N. et al. (1997) J. Biol. Chem. 272, 25913-25919.
4. Ross, S.E. et al. (1999) Mol. Cell. Biol. 19, 8433-8441.

注意事项：

*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:200†
 Unmasking buffer: Citrate
 Antibody diluent: SignalStain® Antibody Diluent #8112
 Detection reagent: SignalStain® Boost (HRP, Rabbit) #8114
†Optimal IHC dilutions determined using SignalStain® Boost IHC
Detection Reagent.
Immunofluorescence (IF-IC) 1:100
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Applications Species Cross-Reactivity* Molecular Wt. Isotype
Entrez-Gene ID #1050
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