DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigmas Anti-FLAG? M2 Antibody)

• 产品编号：CST-8146S      品牌：CST       原厂货号：8146S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

DYKDDDDK Tag (9A3) Mouse mAb能检测细胞中外源表达的DYKDDDDK蛋白。该抗体能识别DYKDDDDK肽，与Sigma's Anti-FLAG® antibodies识别的位点一样。单克隆抗体通过用DYKDDDDK合成肽免疫动物制备。附加表位对于用免疫印迹、免疫沉淀和免疫染色技术来进行的蛋白质标记和检测很有用。因为它们很小，不大可能会影响被标记蛋白的生化特性。DYKDDDDK肽作为一个普通的附加表位广泛地应用于表达载体中。此肽能作为相关蛋白的氨基端或羧基端融合蛋白进行表达和检测（1）。

1. Brizzard, B.L. et al. (1994) Biotechniques 16, 730-5.

原厂资料：

Specificity / Sensitivity

DYKDDDDK Tag (9A3) Mouse mAb detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma's Anti-FLAG® antibodies.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).

1. Brizzard, B.L. et al. (1994) Biotechniques 16, 730-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody