PDLIM2 Antibody

• 产品编号：CST-8144S      品牌：CST       原厂货号：8144S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PDLIM2 Antibody识别内源性PDLIM2总蛋白。通过人工合成人源PDLIM2蛋白Gly255位点相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。PDLIM2蛋白又称为Mystique，包含一个氨基端PDZ区域和一个羧基端LIM区域。PDLIM2蛋白起初被发现在上皮细胞中与细胞骨架蛋白有关联，这将促进细胞粘附和迁移(1,2)。随后的研究发现PDLIM2蛋白也能够通过作为p65的一个细胞核泛素E3连接酶抑制NF-κB的活性 (3)。在癌症细胞系中，PDLIM2蛋白通过DNA甲基化被抑制(4,5)。PDLIM2蛋白的表达能够抑制不依赖支持物生长和肿瘤形成。

1. Torrado, M. et al. (2004) Invest Ophthalmol Vis Sci 45, 3955-63.
2. Loughran, G. et al. (2005) Mol Biol Cell 16, 1811-22.
3. Tanaka, T. et al. (2007) Nat Immunol 8, 584-91.
4. Qu, Z. et al. (2010) Cancer Res 70, 1766-72.
5. Qu, Z. et al. (2010) J Biol Chem 285, 11786-92.

原厂资料：

Specificity / Sensitivity

PDLIM2 Antibody recognizes endogenous levels of total PDLIM2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly255 of human PDLIM2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

PDLIM2, also known as Mystique, contains an amino-terminal PDZ domain and a carboxy-terminal LIM domain. PDLIM2 was orginally found to be associated with cytoskeletal proteins in epithelial cells to promote cell attachment and migration (1,2). Subsequent studies have shown that PDLIM2 can also inhibit NF-κB activity by acting as a nuclear ubiquitin E3 ligase for p65 (3). PDLIM2 is suppressed in cancer cell lines by DNA methylation (4,5). Expression of PDLIM2 can inhibit anchorage-independent growth and tumor formation.

1. Torrado, M. et al. (2004) Invest Ophthalmol Vis Sci 45, 3955-63.
2. Loughran, G. et al. (2005) Mol Biol Cell 16, 1811-22.
3. Tanaka, T. et al. (2007) Nat Immunol 8, 584-91.
4. Qu, Z. et al. (2010) Cancer Res 70, 1766-72.
5. Qu, Z. et al. (2010) J Biol Chem 285, 11786-92.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.