NRBF-2 Antibody

• 产品编号：CST-8066S      品牌：CST       原厂货号：8066S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NRBF-2 Antibody识别内源性水平的总NRBF-2蛋白。该抗体可能与NRBF-1/COPR-1发生交叉反应。该多克隆抗体通过用合成肽免疫动物制备，该合成肽是人NRBF-2蛋白亮氨酸（145位）附近的残基。抗体由蛋白A和肽亲和层析纯化。核受体结合因子-2（NRBF-2）也被称为PPAR和RXRα-2（COPR-2）的共调节子，已被证明其与一些核激素受体AF-2区域有不同的亲和力，如PPARα、RARα、 RARγ 和RXRα（1,2）。 NRBF-2包含一个LLYLL模体，与LXXLL NR框相匹配，对于功能性NRBF-2/核受体复合物的形成和抑制受体功能是需要的。NRBF-2还包含一个独特的自主激活结构域，因此不能完全去除核受体的功能，这表明NRBF-2可能作为一个分子可变电阻器，以微调已配位核受体的转录活性（1,2）。

1. Yasumo, H. et al. (2000) Biochim Biophys Acta 1490, 189-97.
2. Flores, A.M. et al. (2004) J Invest Dermatol 123, 1092-101.

原厂资料：

Specificity / Sensitivity

NRBF-2 Antibody recognizes endogenous levels of total NRBF-2 protein. This antibody may cross-react with NRBF-1/COPR-1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu145 of human NRBF-2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nuclear Receptor Binding Factor-2 (NRBF-2), also referred to as Comodulator of PPAR and RXRα-2 (COPR-2), has been shown to interact with the AF-2 region of several nuclear hormone receptors with varying affinities such as PPARα, RARα, RARγ, and RXRα (1,2). NRBF-2 contains a LLYLL motif, which matches the LXXLL NR box consensus and is required for functional NRBF-2/nuclear receptor complex formation and repression of receptor function. NRBF-2 also contains a unique autonomous activation domain and thus, does not completely abrogate nuclear receptor function, suggesting that NRBF-2 might serve as a molecular rheostat to fine-tune the transcriptional activity of liganded nuclear receptors (1,2).

1. Yasumo, H. et al. (2000) Biochim Biophys Acta 1490, 189-97.
2. Flores, A.M. et al. (2004) J Invest Dermatol 123, 1092-101.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot
the antibody