Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb

• 产品编号：CST-8053S      品牌：CST       原厂货号：8053S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb兔单抗只能检测内源的在Thr102 和/或 Thr104位点磷酸化的JunB蛋白。此抗体不与非磷酸化的JunB蛋白交叉反应。此单克隆抗体是通过合成人源对应的JunB Thr102和Thr104位点周围的磷肽段来免疫动物而获得。JunB是一个碱性亮氨酸拉链(bZIP)转录因子，属于Jun家族，能够诱导c-Jun和JunD。Jun家族的成员与Fos 和ATF蛋白形成具有转录因子功能同源或异源二聚体AP-1(激活蛋白1)，其活性通过各种生理和病理的刺激如生长因子，感染和胁迫信号(1-4)。JunB有时能够对抗c-Jun转录活性,在小鼠的发育中，它可能在功能上取代c-jun (5-7)。JunB调控造血干细胞数目并在慢性骨髓性白血病的发病机理(CML)和急性骨髓性白血病(AML)中起了重要的作用(8,9)。在T细胞的分化中，JunB选择性的在T细胞的助细胞2(Th2)中诱导表达。JunB与c-Maf相互作用形成复合体并协同作用从而激活白介素4(IL-4)的转录,这是Th2所分泌的特征因子之一。对IL-4的转录调控通过JNK介导的在JunB Thr102和Thr104位点的磷酸化而得到加强(10)。在这些位点的磷酸化加强了DAN与JunB/c-Maf复合体在IL-4启动子的P1调控位点的结合，从而导致了Th2限制了IL-4的表达。

1. Busch, S.J. and Sassone-Corsi, P. (1990) Trends Genet. 6, 36-40.
2. Shaulian, E. and Karin, M. (2002) Nat. Cell Biol. 4, E131-E136.
3. Hess, J. et al. (2004) J. Cell Sci. 117, 5965-5973.
4. Mechta-Grigoriou, F. et al. (2001) Oncogene 20, 2378-2389.
5. Chiu, R. et al. (1989) Cell 59, 979-986.
6. Schütte, J. et al. (1989) Cell 59, 987-997.
7. Passegué, E. et al. (2002) Nat. Genet. 30, 158-166.
8. Steidl, U. et al. (2006) Nat. Genet. 38, 1269-1277.
9. Passegué, E. et al. (2004) Cell 119, 431-443.
10. Li, B. et al. (1999) EMBO J 18, 420-32.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb recognizes endogenous levels of JunB protein when phosphorylated at Thr102 and/or Thr104. The antibody does not detect non-phosphorylated JunB protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Thr102 and Thr104 of human JunB protein.

Background

JunB is a basic region, leucine zipper (bZIP) transcription factor belonging to the Jun family that includes c-Jun and JunD. Jun family members homodimerize or heterodimerize with Fos and ATF proteins to form a functional transcription factor AP-1 (activator protein 1), whose activity is regulated by a variety of physiological and pathological stimuli such as growth factors, infections, and stress signals (1-4). While JunB sometimes antagonizes c-Jun transcriptional activity, it may functionally substitute for c-Jun during development in mice (5-7). JunB regulates hematopoietic stem cell number and plays an important role in the pathogenesis of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) (8,9).JunB expression is selectively induced in T helper 2 (Th2) cells during T cell differentiation. JunB interacts with c-Maf, and the resulting complex functions synergistically to activate transcription of Interleukin-4 (IL-4), one of the signature cytokines secreted by Th2 cells. Transcriptional regulation of IL-4 was shown to be enhanced by JNK-mediated phosphorylation of JunB at Thr102 and Thr104 (10). Phosphorylation of these residues enhances DNA binding of the JunB/c-Maf complex at the P1 regulatory site of the IL-4 promoter, leading to Th2-restricted IL-4 expression.

1. Busch, S.J. and Sassone-Corsi, P. (1990) Trends Genet. 6, 36-40.
2. Shaulian, E. and Karin, M. (2002) Nat. Cell Biol. 4, E131-E136.
3. Hess, J. et al. (2004) J. Cell Sci. 117, 5965-5973.
4. Mechta-Grigoriou, F. et al. (2001) Oncogene 20, 2378-2389.
5. Chiu, R. et al. (1989) Cell 59, 979-986.
6. Schütte, J. et al. (1989) Cell 59, 987-997.
7. Passegué, E. et al. (2002) Nat. Genet. 30, 158-166.
8. Steidl, U. et al. (2006) Nat. Genet. 38, 1269-1277.
9. Passegué, E. et al. (2004) Cell 119, 431-443.
10. Li, B. et al. (1999) EMBO J 18, 420-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.