Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-8009S      品牌：CST       原厂货号：8009S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (Alexa Fluor® 647 Conjugate) 只能检测内源的在Tyr701位点磷酸化的Stat1α 和 Stat1β蛋白。此抗体不与对应的酪氨酸磷酸化的Stat家族的其它蛋白交叉反应。此单克隆抗体是通过合成人源对应的Stat1 Tyr701位点周围的磷肽段来免疫动物而获得。CST公司生产的此抗体与Alexa Fluor® 647荧光染料偶联，并通过人源细胞的流式细胞仪的测试。正如预期，Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (Alexa Fluor® 647 Conjugate)与Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167抗体相同，都能够与同物种中的phospho-Stat1蛋白交叉反应。转录因子Stat1的激活是通过一系列的配体完成 (1) 这对IFN-α和 IFN-γ的响应非常重要(2,3)。 在 Tyr701位点的磷酸化诱导 Stat1的形成二聚体, 入核和DNA结合(4)。 Stat1 蛋白是以成对的形式存在Stat1α (91 kDa) 和 另外的剪切方式Stat1β (84 kDa)。在很多细胞中，这两种形式都是通过IFN-α激活。但是有时 Stat1α是被 IFN-γ激活。 Stat1这种不适当的激活方式在存在于很多肿瘤中(5)。除了酪氨酸磷酸化，Stat1 也能通过p38有丝分裂激活蛋白激酶依赖的通路在Ser727位点磷酸化而激活，从而响应IFN-α 和其它的细胞胁迫(6)。丝氨酸磷酸化对最大化的诱导Stat1介导的基因激活也是必需的。

1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Frank, D.A. (1999) Mol Med 5, 432-56.
6. Wen, Z. et al. (1995) Cell 82, 241-50.

原厂资料：

Specificity / Sensitivity

Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of Stat1 only when phosphorylated at Tyr701. The antibody detects phosphorylated Tyr701 of both Stat1α and Stat1β. It does not cross-react with the corresponding phospho-tyrosines of other Stat proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr701 of human Stat1 protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167.

Background

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Frank, D.A. (1999) Mol Med 5, 432-56.
6. Wen, Z. et al. (1995) Cell 82, 241-50.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4ºC. Protect from light. Do not
freeze.