PathScan? Total α-Tubulin Sandwich ELISA Antibody Pair

• 产品编号：CST-7945S      品牌：CST       原厂货号：7945S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

其它组分：

描述：

对于抗体对的specificity和sensitivity，请参考相应的PathScan® Sandwich ELISA Kit。说明：因为通过内部实验确定，该试剂盒检测已知的物种，但是可能也会检测其它物种的同源性蛋白。CST's PathScan® Total α-Tubulin Sandwich ELISA Antibody Pair被提供作为PathScan® Total α-Tubulin Sandwich ELISA Kit #7944中一个十分经济产品。吸附和检测的抗体(100X stocks)以及HRP-conjugated secondary antibody (1000X stock)被提供。对于4 x 96 well ELISAs，充足的试剂被提供。α-tubulin rabbit在PBS中包被在微孔板上过夜。在封闭后，细胞裂解液加入之后是一个α-tubulin mouse detection antibody和HRP-linked, anti-mouse IgG antibody加入。HRP底物TMB被加入后显色。该显色的吸光度值的大小与α-tubulin蛋白量成比例。试剂盒里的抗体是针对该试剂盒特定制备的。细胞骨架由三种类型的细胞质基质纤维组成：微管、微丝(肌动蛋白纤维)和中间纤维。球形微管蛋白亚基包含微管建筑块，由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。γ-tubulin被需要在tubulin亚单位的成核聚合作用中去形成微管聚合物。许多细胞运动是通过微管动力介导的，包括纤毛和鞭毛的跳动、膜泡的核质转运、在减数分裂/有丝分裂期间染色体排布和神经轴突的迁移。这些运动由于竞争性微管聚合作用和解聚作用或通过微管动力蛋白的运动(1)。

1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Total α-Tubulin Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total α-Tubulin Sandwich ELISA Kit #7944. Capture and detection antibodies (100X stocks) and an HRP-linked secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The α-tubulin rabbit capture antibody is coated in PBS overnight onto a 96 well microplate. After blocking, cell lysate is added followed by an α-tubulin mouse detection antibody and HRP-linked, anti-mouse IgG antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of α-tubulin. Microtubule stabilizing or destabilizing agents may significantly increase or decrease the signal, respectively.

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

注意事项：

Storage: Capture and detection antibodies are stored at 4ºC.
HRP-linked, anti-mouse IgG antibody is stored at -20ºC.