PathScan? Total β-Actin Sandwich ELISA Antibody Pair

• 产品编号：CST-7881S      品牌：CST       原厂货号：7881S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

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描述：

对于抗体对的specificity和sensitivity，请参阅相应的PathScan® Sandwich ELISA Kit。说明：因为通过内部实验确定，该抗体对检测已表明的物种，但是可能也会检测其它物种的同源性蛋白。CST's PathScan® Total β-Actin Sandwich ELISA Antibody Pair被提供作为PathScan® Total β-Actin Sandwich ELISA Kit #7880中一个十分经济产品。吸附和检测的抗体(100X stocks)以及HRP-conjugated secondary antibody (1000X stock)被提供。对于4 x 96 well ELISAs，充足的试剂被提供。β-actin rabbit capture antibody在PBS中包被在微孔板上过夜。在封闭后，细胞裂解液加入之后是一个pan-actin mouse detection antibody和HRP-linked, anti-mouse IgG antibody加入。HRP底物TMB被加入后显色。该显色的吸光度值的大小与β-actin蛋白量成比例。试剂盒里的抗体是针对该试剂盒特定制备的。Actin是一个广泛存在的真核细胞蛋白质，其实细胞骨架的重要组成成分。在哺乳类动物中至少有六种亚型。非肌肉组织β-和γ-actin也称作细胞质actin，它们在非肌肉组织中主要的表达，目的是控制细胞结构和运动(1)。α-cardiac和α-skeletal actin分别在心脏横纹肌和骨骼肌表达；两个平滑肌actins（α-和γ-actin）各自主要地发现在血管平滑肌和肠道平滑肌。这些actin亚型调节肌肉细胞的收缩潜能(1)。Actin主要存在作为一个纤维聚合物F-actin。在一些进程例如细胞分裂、内吞作用或应激中回应细胞骨架重组信号，cofilin促进分裂和F-actin的解聚，从而导致在单体球状形式G-actin的增加(2)。Arp2/3复合物使F-actin片段稳定，并且促进新的actin细丝形成(2)。有研究报道actin是在主要乳腺肿瘤中高度磷酸化(3)。在凋亡条件下actin的分裂已经在体外、心肌和骨骼肌中观察(4-6)。通过caspase-3剪切的Actin可能加速泛素/蛋白酶体依赖的肌肉蛋白水解(6)。

1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
3. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
4. Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
5. Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
6. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Total β-Actin Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total β-Actin Sandwich ELISA Kit #7880. Capture and detection antibodies (100X stocks) and an HRP-linked secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The β-actin rabbit capture antibody is coated in PBS overnight onto a 96 well microplate. After blocking, cell lysate is added followed by pan-actin mouse detection antibody and HRP-linked, anti-mouse IgG antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of β-actin.

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6).

1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
3. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
4. Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
5. Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
6. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

注意事项：

Storage: β-actin-specific rabbit capture and pan-actin-specific
mouse detection antibodies are stored at 4ºC. HRP-linked, anti-
mouse IgG antibody is stored at -20ºC