AFP (3H8) Mouse mAb (Alexa Fluor? 594 Conjugate)

• 产品编号：CST-7877S      品牌：Cell Signaling Technology       原厂货号：7877S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

AFP (3H8)Mouse mAb（Alexa Fluor^® 594偶联）可以识别内源性的总AFP蛋白。合成人AFP蛋白的重组蛋白片段免疫动物获得单克隆抗体。Cell Signaling Technology公司抗体偶联Alexa Fluor^® 594荧光素染料，并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联Alexa Fluor^® 594的AFP (3H8)#3903显示同样的物种交叉反应。Alpha-fetoprotein (AFP)是一个在哺乳动物胎儿肝脏，卵黄囊,和胃肠道中发现的65kDa的糖蛋白。AFP在成体细胞中表达较低，而在成体肝癌细胞中异常表达（1,2）。抑癌基因p53和β-catenin都涉及到对AFP的表达调控。在正常成体细胞中，p53和AFP基因的抑制区结合，因此阻断其转录。P53和β-catenin的突变都与AFP的异常表达相关。研究显示AFP在血清中表达升高预示着肝细胞癌（3）。

1. Meier, V. et al. (2006) Comp Hepatol 5, 2.
2. Warnes, T.W. and Smith, A. (1987) Baillieres Clin Gastroenterol 1, 63-89.
3. Peng, S.Y. et al. (2004) Int J Cancer 112, 44-50.

原厂资料：

Specificity / Sensitivity

AFP (3H8) Mouse mAb (Alexa Fluor^® 594 Conjugate) recognizes endogenous levels of total AFP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human AFP.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated AFP (3H8) Mouse mAb #3903.

Background

Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).

1. Meier, V. et al. (2006) Comp Hepatol 5, 2.
2. Warnes, T.W. and Smith, A. (1987) Baillieres Clin Gastroenterol 1, 63-89.
3. Peng, S.Y. et al. (2004) Int J Cancer 112, 44-50.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the anti-
body. Protect from light. Do not freeze.