Cre Recombinase Antibody

• 产品编号：CST-7803S      品牌：Cell Signaling Technology       原厂货号：7803S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cre Recombinase Antibody能识别转染水平的总Cre重组酶蛋白。多克隆抗体通过用合成肽免疫动物制备，该合成肽是Cre重组酶蛋白Asp141周围的残基。抗体用蛋白A以及多肽亲和色谱法纯化。Cre重组酶是一种细菌噬菌体P1酶，是在溶源状态下噬菌体基因组维持单体质粒所必需的（1,2）。该酶介导两个有34个碱基对的loxP位点之间的位点专一重组。这个反应可以在体外发生，说明它不需要辅助因子（3）。Cre/Lox系统应用于许多体内和体外研究，包括靶基因缺失（4）和基因特异性的人源化动物模型（5）。Cre-Lox复合物晶体结构的分辨率显示两个Cre分子与一个Lox位点相互作用（6）。

1. Abremski, K. et al. (1983) Cell 32, 1301-11.
2. Sternberg, N. et al. (1981) Cold Spring Harb Symp Quant Biol 45 Pt 1, 297-309.
3. Abremski, K. and Hoess, R. (1984) J Biol Chem 259, 1509-14.
4. Qin, M. et al. (1994) Proc Natl Acad Sci U S A 91, 1706-10.
5. Lakso, M. et al. (1992) Proc Natl Acad Sci U S A 89, 6232-6.
6. Guo, F. et al. (1997) Nature 389, 40-6.

原厂资料：

Specificity / Sensitivity

Cre Recombinase Antibody recognizes transfected levels of total Cre recombinase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp141 of Cre recombinase protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34 base pair loxP sites. This reaction can be carried out in vitro, indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitro and in vivo applications including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6).

1. Abremski, K. et al. (1983) Cell 32, 1301-11.
2. Sternberg, N. et al. (1981) Cold Spring Harb Symp Quant Biol 45 Pt 1, 297-309.
3. Abremski, K. and Hoess, R. (1984) J Biol Chem 259, 1509-14.
4. Qin, M. et al. (1994) Proc Natl Acad Sci U S A 91, 1706-10.
5. Lakso, M. et al. (1992) Proc Natl Acad Sci U S A 89, 6232-6.
6. Guo, F. et al. (1997) Nature 389, 40-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody