PathScan? Total p27 Kip1 Sandwich ELISA Kit

• 产品编号：CST-7768C      品牌：Cell Signaling Technology       原厂货号：7768C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

PathScan® Total p27 Kip1 Sandwich ELISA Kit 能够检测内源性 p27 Kip1总蛋白。本试剂盒能够检测通过内部测试的特定种属蛋白，但也许能够检测其他种属的同源蛋白。CST's PathScan® Total p27 Kip1 Sandwich ELISA Kit为固相三明治酶联免疫吸附试剂盒，能够检测内源性p27 Kip1蛋白。p27 Kip1 Rabbit Antibody包被于微孔上。与细胞裂解液孵育后，p27 Kip1被包被抗体捕获。多次洗涤后，加入p27 Kip1 Mouse Detection Antibody检测被捕获的p27 Kip1蛋白。随后，用Anti-mouse IgG, HRP-linked Antibody识别结合的检测抗体。加入HRP底物(TMB)进行显色。显色过程中吸光度的强度和p27 Kip1蛋白的量成正比。试剂盒内的抗体具有针对试剂盒的固定搭配。p27 Kip1是周期蛋白依赖性激酶抑制剂Cip/Kip家族的一员。同其类似物一样，如p57 Kip2 和p21 Waf1/Cip1，其巩固G1限制点的能力源于CDK2/cyclin E和其它CDK/cyclin复合体的结合抑制。在静止期细胞和cAMP或其它细胞周期阴性调节剂处理的细胞中，p27的表达是上调的。白细胞介素2或者其它有丝分裂原能够诱导p27的下调；该过程涉及p27的磷酸化和泛素化-蛋白酶体途径介导的降解(1-4)。

1. Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23.
2. Polyak, K. et al. (1994) Genes Dev 8, 9-22.
3. Kato, J.Y. et al. (1994) Cell 79, 487-96.
4. Vlach, J. et al. (1997) EMBO J 16, 5334-44.

原厂资料：

Specificity / Sensitivity

PathScan® Total p27 Kip1 Sandwich ELISA Kit detects endogenous levels of total p27 Kip1 protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Total p27 Kip1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of p27 Kip1. A p27 Kip1 Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, p27 Kip1 protein is captured by the coated antibody. Following extensive washing, a p27 Kip1 Mouse Detection Antibody is added to detect the captured p27 Kip1 protein. Anti-Mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of p27 Kip1 protein. 
 Antibodies in kit are custom formulations specific to kit

Background

p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).

1. Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23.
2. Polyak, K. et al. (1994) Genes Dev 8, 9-22.
3. Kato, J.Y. et al. (1994) Cell 79, 487-96.
4. Vlach, J. et al. (1997) EMBO J 16, 5334-44.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately)