Rabex-5 (D21F12) Rabbit mAb

• 产品编号：CST-7622S      品牌：CST       原厂货号：7622S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rabex-5 (D21F12) Rabbit mAb兔单抗识别内源性的Rabex-5总蛋白水平。该单克隆抗体用与人类Rabex-5蛋白中羧基末端附近的氨基酸序列对应的人工合成肽段免疫动物而制成。Rabex-5也称为RabGEF1和RAP1，被确定为Rab5的鸟嘌呤核苷酸交换因子（GEF），是小Rab GTP酶Ras超家族的成员之一（1）。Rabex-5从产生GTP结合的活性形式的Rab5，并与其效应蛋白Rabaptin-5形成紧密连接（2）。这个复合物定位到内体膜，作为早期内吞中囊泡转运的关键调节者发挥作用（3,4）。Rabex-5也可以被单泛素化，并且有泛素连接酶活性以便被招募到早期内体（5,6）。Rab5在GTP/GDP状态下的构象改变对其生物学功能至关重要，因为其是内吞作用多个步骤中的一个速率限制调节者（5）。通过其对内体转运和入胞的控制，Rabex-5显示出对NGF介导的神经突生长和FcεRI依赖的肥大细胞活化的负向调节（8）。

1. Horiuchi, H. et al. (1997) Cell 90, 1149-59.
2. Lippé, R. et al. (2001) Mol Biol Cell 12, 2219-28.
3. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
4. van der Bliek, A.M. (2005) Nat Cell Biol 7, 548-50.
5. Mattera, R. et al. (2006) J Biol Chem 281, 6874-83.
6. Mattera, R. and Bonifacino, J.S. (2008) EMBO J 27, 2484-94.
7. Liu, J. et al. (2007) Mol Biol Cell 18, 1375-84.
8. Tam, S.Y. et al. (2004) Nat Immunol 5, 844-52.

原厂资料：

Specificity / Sensitivity

Rabex-5 (D21F12) Rabbit mAb recognizes endogenous levels of total Rabex-5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rabex-5 protein.

Background

Rabex-5, also called RabGEF1 and RAP1, was identified as a guanine nucleotide exchange factor (GEF) for Rab5, a member of the Ras superfamily of small Rab GTPases (1). Rabex-5 generates the GTP-bound active form of Rab5 and forms a tight association with its effector protein Rabaptin-5 (2). This complex localizes to endosomal membranes where it functions as a key regulator of vesicular trafficking during early endocytosis (3,4). Rabex-5 is also monoubiquitinated and has ubiquitin ligase activity that regulates its recruitment to early endosomes (5,6). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis (5). Through its control of endosomal trafficking and endocytosis, Rabex-5 has been shown to negatively regulate NGF-mediated neurite outgrowth (7) as well as FcεRI-dependent mast cell activation (8

1. Horiuchi, H. et al. (1997) Cell 90, 1149-59.
2. Lippé, R. et al. (2001) Mol Biol Cell 12, 2219-28.
3. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
4. van der Bliek, A.M. (2005) Nat Cell Biol 7, 548-50.
5. Mattera, R. et al. (2006) J Biol Chem 281, 6874-83.
6. Mattera, R. and Bonifacino, J.S. (2008) EMBO J 27, 2484-94.
7. Liu, J. et al. (2007) Mol Biol Cell 18, 1375-84.
8. Tam, S.Y. et al. (2004) Nat Immunol 5, 844-52.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.