Puma Antibody (Rodent Specific)

• 产品编号：CST-7467S      品牌：CST       原厂货号：7467S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Puma Antibody (Rodent Specific) 能够检测内源性水平的小鼠和大鼠Puma总蛋白。该多克隆抗体是采用合成的小鼠源Puma蛋白Pro75周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。Puma（P53上调凋亡调节子） ，是一个“BH3-only” Bcl-2家族蛋白，最初确定为一个p53诱导的差异表达基因（1,2） 。 “BH3-only”家族成员包括Bad、Bik、Bid、Bim、Hrk和Noxa，所有这些都包含BH3结构域，但缺乏其他的保守结构域，BH1和BH2 ，通过BH3结构域的相互作用结合或拮抗Bcl-2抗凋亡家族成员，进而促进细胞凋亡（3）。puma基因产生两个含BH3的蛋白质Puma-α和Puma-β，这两者都是由p53诱导产生，结合Bcl-2和Bcl-xL，定位到线粒体，促进细胞色素c的释放和细胞凋亡（1,2）。Puma在p53肿瘤抑制途径中起着至关重要的作用。靶向性地破坏Puma基因，损害p53介导的细胞凋亡和肿瘤抑制（4-7）。研究表明，Puma基因敲除小鼠具有多凋亡刺激缺陷，包括电离辐射、 c-Myc基因表达失调和细胞因子停止（4）。

1. Yu, J. et al. (2001) Mol Cell 7, 673-82.
2. Nakano, K. and Vousden, K.H. (2001) Mol Cell 7, 683-94.
3. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74.
4. Jeffers, J.R. et al. (2003) Cancer Cell 4, 321-8.
5. Hemann, M.T. et al. (2004) Proc Natl Acad Sci U S A 101, 9333-8.
6. Yu, J. et al. (2003) Proc Natl Acad Sci U S A 100, 1931-6.
7. Villunger, A. et al. (2003) Science 302, 1036-8.

原厂资料：

Specificity / Sensitivity

Puma Antibody (Rodent Specific) recognizes endogenous levels of total Puma protein in mouse and rat.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro75 of mouse Puma protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Puma (p53 upregulated modulator of apoptosis) is a "BH3-only" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the puma gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria and promote cyctochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the puma gene impairs p53-mediated apoptosis and tumor suprression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4).

1. Yu, J. et al. (2001) Mol Cell 7, 673-82.
2. Nakano, K. and Vousden, K.H. (2001) Mol Cell 7, 683-94.
3. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74.
4. Jeffers, J.R. et al. (2003) Cancer Cell 4, 321-8.
5. Hemann, M.T. et al. (2004) Proc Natl Acad Sci U S A 101, 9333-8.
6. Yu, J. et al. (2003) Proc Natl Acad Sci U S A 100, 1931-6.
7. Villunger, A. et al. (2003) Science 302, 1036-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody