Tie2 (D9D10) Rabbit mAb

• 产品编号：CST-7403S      品牌：CST       原厂货号：7403S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Tie2 (D9D10) 兔单抗能够识别内源性水平的总Tie2蛋白。该单克隆抗体通过用合成肽免疫动物制备，该合成肽是人Tie2蛋白精氨酸（580位）附近的残基。Tie2/Tek 是一个几乎仅在内皮细胞上表达的酪氨酸激酶受体 (RTK)。它对于血管生成十分关键，对成年人血管内皮细胞的存活、完整性以及肿瘤血管新生都十分重要(1-3)。血管生成素（angiopoietins）家族的蛋白能作为配体和Tie2结合。有趣的是，这些配体具有相反的作用，比如Angiopoietin-1 (Ang1) 和 Angiopoietin-4 (Ang4)能刺激Tie2酪氨酸位点的磷酸化， 而Angiopoietin-2 (Ang2) 和Angiopoietin-3 (Ang3) 能抑制这种磷酸化(4,5)。下游的信号组分，包括Grb2, Grb7, Grb14, Shp2, PI3K激酶的p85亚基和p56/Dok-2通过它们的SH2或PTB结构域以酪氨酸磷酸化依赖的形式和Tie2相互作用(6,7)。Tyr992定位在Tie2分子的假定活化环状区是一个主要的自磷酸化位点(8)。

1. Ward, N.L. and Dumont, D.J. (2002) Semin. Cell Dev. Biol. 13, 19-27.
2. Jones, N. and Dumont, D.J. (2000) Cancer Metastasis Rev. 19, 13-17.
3. Partanen, J. and Dumont, D.J. (1999) Curr. Top. Microbiol. Immunol. 237, 159-172.
4. Ellis, L. M. et al. (2002) Oncology 16, 31-35.
5. Koh, G. Y. et al. (2002) Exp. Mol. Med. 34, 1-11.
6. Jones, N. et al. (1999) J. Biol. Chem. 274, 30896-30905.
7. Jones, N. et al. (2003) Mol. Cell. Biol. 23, 2658-2668.
8. Murray, B. W. et al. (2001) Biochem. 40, 10243-10253.

原厂资料：

Specificity / Sensitivity

Tie2 (D9D10) Rabbit mAb recognizes endogeneous levels of total Tie2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg580 of human Tie2 protein.

Background

Tie2/Tek is a receptor tyrosine kinase (RTK) expressed almost exclusively on endothelial cells. It is critical for vasculogenesis and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis (1-3). A family of ligands known as the angiopoietins binds to Tie2. Interestingly, these ligands appear to have opposing actions; Angiopoietin-1 (Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2; Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation (4,5). Downstream signaling components, including Grb2, Grb7, Grb14, SHP-2, the p85 subunit of phosphatidylinositol 3-kinase, and p56/Dok-2 interact with Tie2 in a phosphotyrosine-dependent manner through their SH2 or PTB domains (6,7). Tyr992 is located on the putative activation loop of Tie2 and is a major autophosphorylation site (8).

1. Ward, N.L. and Dumont, D.J. (2002) Semin. Cell Dev. Biol. 13, 19-27.
2. Jones, N. and Dumont, D.J. (2000) Cancer Metastasis Rev. 19, 13-17.
3. Partanen, J. and Dumont, D.J. (1999) Curr. Top. Microbiol. Immunol. 237, 159-172.
4. Ellis, L. M. et al. (2002) Oncology 16, 31-35.
5. Koh, G. Y. et al. (2002) Exp. Mol. Med. 34, 1-11.
6. Jones, N. et al. (1999) J. Biol. Chem. 274, 30896-30905.
7. Jones, N. et al. (2003) Mol. Cell. Biol. 23, 2658-2668.
8. Murray, B. W. et al. (2001) Biochem. 40, 10243-10253.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.