PathScan? Phospho-MEK1 (Ser217/221) Sandwich ELISA Antibody Pair

• 产品编号：CST-7211S      品牌：CST       原厂货号：7211S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

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描述：

对于Antibody Pair的特异性和灵敏度，请参照相关的PathScan® Sandwich ELISA Kit。注意：经内部测试确认，本抗体对可以检测到所列物种中的蛋白。但对其他物种中的同源蛋白可能也可以检测。CST的PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Antibody Pair是可以替代PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Kit #7175的更经济的产品。其中提供了捕获抗体、检测抗体(100X stocks)和HRP偶联的二抗(1000X stock)。这些试剂足够完成4 x 96 孔板的ELISAs实验。在96孔板中，加入MEK1捕获抗体进行包被。封闭后，加入细胞裂解液，随后分别加入p-MEK1/2 Detection Antibody、anti-Rabbit IgG的HRP偶联抗体。最后加入HRP底物—— TMB，进行显色。产生的吸光值与p-MEK1(Ser217/221）蛋白的数量成正比。*试剂盒中抗体是专为本试剂盒特制的。MEK1、MEK2,也被称为MAPK或Erk激酶，它们是双特异性蛋白激酶，在丝裂原活化蛋白激酶级联反应中发挥作用，控制细胞生长和分化(1-3)。MEK1、MEK2的活化是通过Raf样分子对两个丝氨酸（217 /221位点）的磷酸化而实现的，这两个丝氨酸位点位于亚结构域VIII的活化环内。多种生长因子和细胞因子以及膜去极化和钙内流都能激活MEK1/2(1-4)。组成型活化的MEK1/2能够诱导NIH/3T3细胞的转化和PC-12细胞的分化(4)。MEK蛋白通过将p44、p42 MAPK激酶上位于亚结构域VIII的活化环内的丝氨酸和苏氨酸磷酸化，激活p44、 p42 MAPK激酶。

1. Crews, C.M. et al. (1992) Science 258, 478-480.
2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
3. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
4. Cowley, S. et al. (1994) Cell 77, 841-852.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Kit #7175. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The MEK1 Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by Phospho-MEK1/2 (Ser217/221) Detection Antibody and anti-Rabbit IgG, HRP-conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-MEK1/2 (Ser217/221) protein. 
 *Antibodies in this kit are custom formulations specific to the kit.

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

1. Crews, C.M. et al. (1992) Science 258, 478-480.
2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
3. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
4. Cowley, S. et al. (1994) Cell 77, 841-852.

注意事项：

Storage: MEK1 Capture and Phospho-MEK1/2
(Ser217/221) Detection Antibodies are stored at 4oC.
Anti-Rabbit IgG, HRP-Linked Antibody is stored at -20oC.