PathScan? Phospho-Histone H3 (Ser10) Sandwich ELISA Antibody Pair

• 产品编号：CST-7207S      品牌：CST       原厂货号：7207S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
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描述：

对于抗体对specificity和sensitivity，请参考相应的PathScan® Sandwich ELISA Kit。说明：经内部测试确认，本试剂盒可以检测到所列物种中的蛋白。但对其他物种中的同源蛋白可能也可以检测。CST's PathScan® Phospho-Histone H3 (Ser10) Sandwich ELISA Antibody Pair可以作为PathScan® Phospho-Histone H3 (Ser10) Sandwich ELISA Kit #7155中的一个可选择产品。提供了吸附和检测的抗体(100X stocks)以及HRP偶联的二抗(1000X stock)。对于4 x 96 well ELISAs也提供了充足的试剂。Histone Capture Antibody在PBS中包被在微孔板上过夜。封闭后，细胞裂解液加入之后是一个Biotinylated Phospho-Histone H3 (Ser10) Detection Antibody和HRP-conjugated Streptavidin加入。HRP底物TMB被加入后显色。在450 nm处产生的吸光度值与Phospho-Histone H3 (Ser10)蛋白数量成正比。*试剂盒中抗体是专为本试剂盒特制的。染色质结构的修饰在调节真核细胞的转录中扮演着重要的角色。由DNA围绕和八聚体组蛋白（H2A，H2B，H3和H4各两个）共同组成的核小体是染色质的主要组成（1）。核小体的组蛋白氨基酸尾端进行不同的转录后修饰，包括乙酰化，磷酸化，甲基化和泛素化（2-5）。这些修饰通过不同的刺激产生并且对转录因子能否接近染色质有着直接的影响，所以也影响着基因的表达（6）。在大多数的物种中组蛋白H2B主要在Lys5,，12,，15和20位点上发生乙酰化（4,7）。组蛋白H3主要是在Lys9，14，18，23，27和56位点上发生乙酰化。在某些物种里H3上的Lys9位点发生乙酰化并应该在组蛋白沉积和染色质组装中扮演着重要的角色（2,3）。组蛋白H3上Ser10，Ser28和Thr11位点的磷酸化在有丝分裂和无丝分裂中都与染色质的缩合紧密相连（8-10）。H3的Thr3位点的磷酸化在许多物种中都是高度保守的，是由kinase haspin所催化的。在哺乳动物中用磷酸化特异性的抗体做免疫组化显示H3的Thr3位点在有丝分裂的前期发生磷酸化，后期发生去磷酸化（11）。

1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Phospho-Histone H3 (Ser10) Sandwich ELISA Antibody Pair is being offered as an alternative to our PathScan® Phospho-Histone H3 (Ser10) Sandwich ELISA Kit #7155. Capture and Detection antibodies (100X stocks) and HRP-conjugated Streptavidin (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. Histone Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by a Biotinylated Phospho-Histone H3 (Ser10) Detection Antibody and HRP-conjugated Streptavidin. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of Phospho-Histone H3 (Ser10) protein. 
 *Antibodies in this kit are custom formulations specific to the kit.

Background

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

注意事项：

Storage: Histone H3 Capture Antibody and Biotinylated
Phospho-Histone H3 (Ser10) Detection Antibody are stored at
4°C. HRP-Linked Streptavidin is stored at -20°C