PathScan? Phospho-HSP27 (Ser82) Sandwich ELISA Kit

• 产品编号：CST-7152C      品牌：CST       原厂货号：7152C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

其它组分：

描述：

CST's PathScan®Phospho-HSP27 (Ser82) Sandwich ELISA Kit可识别内源性水平的磷酸化HSP27（Ser82）蛋白。使用Sandwich ELISA Kit #7152可以检测到紫外光处理后HeLa细胞中诱导的磷酸化HSP27（Ser82）。该试剂盒经过内部测试，可识别指定物种的蛋白质，也可能识别一些其它物种的同源性蛋白。CST's PathScan® Phospho-HSP27 (Ser82) Sandwich ELISA Kit是一个固相夹心酶联免疫吸附分析（ELISA）试剂盒，可以用来检测内源性的磷酸化HSP27（Ser82）蛋白。HSP27鼠单抗已经被包被于微孔中。在和细胞裂解液孵育后，HSP27被包被的抗体捕获。在充分冲洗后，Phospho-HSP27 (Ser82) Rabbit Antibody被加入以检测被捕获磷酸化HSP27（Ser82）蛋白。抗兔IgG，HRP-连接的抗体#7074，将用来识别结合的的检测抗体。加入HRP底物TMB显色。颜色的吸光程度高低正比于磷酸化HSP27量的多少。热休克蛋白（HSP）27是小HSP之一，在多种细胞和组织中都有不同水平的组成性表达。像其它小HSP一样，HSP27的调节也发生在转录和翻译后（1）。应激反应中，HSP27表达增加了数倍以实现细胞对不利环境的抵抗。HSP27被MAPKAPK-2在Ser15、Ser78和Ser82磷酸化，作为p38 MAP激酶途径活化的结果（2,3）。HSP27磷酸化导致其四级结构改变，以至于从大分子量的同型多聚体变为二聚体和单体（4）。目前显示HSP27磷酸化和浓度增加可以调节肌动蛋白多聚化和再组织（5,6）。

1. Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.
2. Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.
3. Rouse, J. et al. (1994) Cell 78, 1027-1037.
4. Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.
5. Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.
6. Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.

原厂资料：

Specificity / Sensitivity

CST's PathScan® Phospho-HSP27 (Ser82) Sandwich ELISA Kit detects endogenous levels of phospho-HSP27 (Ser82) protein. Using this Sandwich ELISA Kit #7152, a significant induction of phospho-HSP27 (Ser82) can be detected in HeLa cells treated with UV light. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Phospho-HSP27 (Ser82) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-HSP27 (Ser82) protein. An Hsp27 Mouse mAb has been coated onto the microwells. After incubation with cell lysates, HSP27 protein is captured by the coated antibody. Following extensive washing, Phospho-HSP27 (Ser82) Rabbit Antibody is added to detect the captured phospho-HSP27 (Ser82) protein. Anti-rabbit IgG, HRP-linked Antibody #7074 is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of phospho-HSP27 (Ser82) protein.

Description

CST's PathScan® Phospho-HSP27 (Ser82) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-HSP27 (Ser82) protein. An Hsp27 Mouse mAb has been coated onto the microwells. After incubation with cell lysates, HSP27 protein is captured by the coated antibody. Following extensive washing, Phospho-HSP27 (Ser82) Rabbit Antibody is added to detect the captured phospho-HSP27 (Ser82) protein. Anti-rabbit IgG, HRP-linked Antibody #7074 is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of phospho-HSP27 (Ser82) protein.

1. Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.
2. Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.
3. Rouse, J. et al. (1994) Cell 78, 1027-1037.
4. Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.
5. Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.
6. Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).