HIF-2α (D9E3) Rabbit mAb

• 产品编号：CST-7096S      品牌：CST       原厂货号：7096S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HIF-2α (D9E3)Rabbit mAb 兔单抗可以识别内源性的总HIF-2α蛋白。合成与邻近人源HIF-2α蛋白Ala758氨基酸残基序列一致的肽段，免疫动物获得多克隆抗体。缺氧诱导性因子（HIF）是细胞对低氧环境进行反应所必须的（1,2）。在常氧状态下，HIF的α亚基被von Hippel-Lindau (VHL)蛋白泛素化并通过泛素/蛋白酶体途径降解（1,2）。低氧状态能够抑制α亚基的降解从而提高蛋白稳定性（1,2）。HIF随后能够转录调控多种对低氧条件做出反应的基因（1,2）。HIFα亚基有多个亚型（2）。研究表明在人类的某些癌症中HIF-1α 和HIF-2α表达上调。（2）。HIF-1α既能促进又能抑制增殖，而HIF-2α仅有抑制增殖的作用（2）。

1. Kaelin, W.G. (2005) Biochem Biophys Res Commun 338, 627-38.
2. Toschi, A. et al. (2008) J Biol Chem 283, 34495-9.
3. Gordan, J.D. and Simon, M.C. (2007) Curr Opin Genet Dev 17, 71-7.

原厂资料：

Specificity / Sensitivity

HIF-2α (D9E3) Rabbit mAb recognizes endogenous levels of total HIF-2α protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala758 of human HIF-2α protein.

Background

Hypoxia-inducible factor (HIF) is essential for the cellular response to hypoxia (1,2). Under normoxia conditions, the α subunit of HIF is ubiquitinated by von Hippel-Lindau (VHL) protein and is degraded in the ubiquitin/proteasome pathway (1,2). Hypoxia inhibits the degradation of the α subunit, which leads to its stabilization (1,2). HIF, in turn, regulates the transcription of a variety of genes that respond to hypoxia conditions (1,2). There are several isoforms of the HIF α subunit (2). Studies have found that HIF-1α and HIF-2α expression is increased in some human cancers (2). HIF-1α has both pro- and anti-proliferative activities, whereas HIF-2α does not possess anti-proliferative activity (2). Therefore, HIF-2α likely plays an important role in tumorigenesis (2,3).

1. Kaelin, W.G. (2005) Biochem Biophys Res Commun 338, 627-38.
2. Toschi, A. et al. (2008) J Biol Chem 283, 34495-9.
3. Gordan, J.D. and Simon, M.C. (2007) Curr Opin Genet Dev 17, 71-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody