EphA2 (D4A2) XP? Rabbit mAb

• 产品编号：CST-6997P      品牌：Cell Signaling Technology       原厂货号：6997P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

EphA2 的兔源单克隆抗体(D4A2) XP® 能检测内源总 EphA2蛋白的表达水平。单克隆抗体通过用多肽免疫动物得到，该重组多肽是根据人的EphA2蛋白氨基末端序列合成的。Eph受体是研究的最为清楚的酪氨酸激酶受体家族(RTKs)。根据其序列的相似性和一子集配体的倾向性可将它们分为两组：结合基磷脂酰肌醇锚定的麻黄素A配体的EphA受体； 结合具有跨膜区和胞内区的麻黄素B蛋白的EphB 受体(1,2)。Eph 受体及其配体与多种疾病相关，包括肿瘤（3）。Eph A 和B配体都有双重功能。作为RTK配体，麻黄素激活Eph受体的激酶活性以及激活表达该受体细胞的信号通路。麻黄素的胞外区的聚集足以完成这项功能（4）。麻黄素的第二项功能称为“反向信号调节”，凭借胞内区的酪氨酸磷酸化，能够与表达配体的细胞中许多激活信号通路的其它蛋白相互作用（5）。许多刺激都能使麻黄素B的酪氨酸位点磷酸化，包括与麻黄素B受体结合、Src激酶的激活以及受PDGF和 FGF刺激 (6)。酪氨酸 324/327的磷酸化被认为是体内麻黄素 B1的主要磷酸化位点（7）。EphA2高表达于各种肿瘤细胞，它有可能促进恶变。然而也有报道显示EphA2在抑制肿瘤过程中起重要作用（8）。EphA2的促肿瘤作用可能依赖于它的酪氨酸激酶活性。

1. Wilkinson, D.G. (2000) Int Rev Cytol 196, 177-244.
2. Klein, R. (2001) Curr Opin Cell Biol 13, 196-203.
3. Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19, 5614-9.
4. Holder, N. and Klein, R. (1999) Development 126, 2033-44.
5. Brückner, K. et al. (1997) Science 275, 1640-3.
6. Palmer, A. et al. (2002) Mol Cell 9, 725-37.
7. Kalo, M.S. et al. (2001) J Biol Chem 276, 38940-8.
8. Guo, H. et al. (2006) Cancer Res 66, 7050-7058.

原厂资料：

Specificity / Sensitivity

EphA2 (D4A2) XP® Rabbit mAb recognizes endogenous levels of total EphA2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human EphA2 protein. 

Background

The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, the ephrins stimulate the kinase activity of the Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF (6). Tyrosines 324/327 have been identified as major phosphorylation sites of ephrin B1 in vivo (7).

EphA2 is overexpressed in various tumor cells, and it has been suggested that EphA2 may promote malignancy. However, several studies demonstrate that EphA2 plays an important role in tumor suppression (8). The role of EphA2 in tumor development may depend upon regulation of its tyrosine kinase activity.

1. Wilkinson, D.G. (2000) Int Rev Cytol 196, 177-244.
2. Klein, R. (2001) Curr Opin Cell Biol 13, 196-203.
3. Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19, 5614-9.
4. Holder, N. and Klein, R. (1999) Development 126, 2033-44.
5. Brückner, K. et al. (1997) Science 275, 1640-3.
6. Palmer, A. et al. (2002) Mol Cell 9, 725-37.
7. Kalo, M.S. et al. (2001) J Biol Chem 276, 38940-8.
8. Guo, H. et al. (2006) Cancer Res 66, 7050-7058.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.