TACE (D22H4) Rabbit mAb

• 产品编号：CST-6978S      品牌：CST       原厂货号：6978S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TACE (D22H4)Rabbit mAb兔单抗识别内源性的总TACE 蛋白。合成与人源TACE蛋白的氨基末端氨基酸序列一致的肽段，免疫动物获得多克隆抗体。TACE(肿瘤坏死因子-α转化酶),也叫ADAM17，是跨膜非金属蛋白酶，在细胞脱落“shedding"过程中，其功能是剪切众多细胞表面分子。TACE充足表达于多种成人组织，但在胎儿发育表达被差异调控（1）。TACE的一个重要底物是前TNF-α(1)。TACE的增加表达与一系列病理条件包括骨关节炎和风湿性关节炎有关，在那里增加的TNF-α的促炎性效应可促进疾病的病理学（2,3）。最近报道了TACE的其他重要的分子调控，如EGFR配体和Notch。TACE调控了EGFR配体蛋白, 如双调蛋白 和TNF-α的脱落。一些肿瘤因TNF-α的上调和TACE的上调高度激活EGFR,这使得其成为潜在的药物治疗靶标（4）。TACE激活Noctch在配体依赖的方式，已显示在果蝇神经系统发育中的作用（5）TACE也被认为对淀粉样前蛋白起α分泌酶作用（APP）(6)，并与神经干细胞的自我更新和增殖过程有关（7）.

1. Black, R.A. et al. (1997) Nature 385, 729-33.
2. Amin, A.R. (1999) Osteoarthritis Cartilage 7, 392-4.
3. Patel, I.R. et al. (1998) J Immunol 160, 4570-9.
4. Kenny, P.A. (2007) Differentiation 75, 800-8.
5. Delwig, A. and Rand, M.D. (2008) Cell Mol Life Sci 65, 2232-43.
6. Deuss, M. et al. (2008) Curr Alzheimer Res 5, 187-201.

原厂资料：

Specificity / Sensitivity

TACE (D22H4) Rabbit mAb recognizes endogenous levels of total TACE protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TACE protein.

Background

TACE (TNF-α converting enzyme), also known as ADAM17, is a transmembrane metalloprotease that plays a key role in the cleavage of a number cell surface molecules in a process known as “shedding". TACE is abundantly expressed in many adult tissues, but in fetal development expression is differentially regulated (1). An important substrate of TACE is pro-TNF-α (1). Increased expression of TACE is associated with several pathological conditions including osteoarthritis and rheumatoid arthritis, where the pro-inflammatory effects of increased TNF-α contribute to disease pathogenesis (2,3). Regulation of other important molecules by TACE such as EGFR and Notch has recently been documented. TACE is responsible for the shedding of EGFR ligands such as amphiregulin and TNF-α. Some tumors have hyperactivated EGFR due to upregulated TNF-α production and upregulated TACE, making TACE a potential target for drug development (4). TACE activates Notch in a ligand-independent manner and has been shown to play a role in the development of the Drosophila nervous system (5). TACE has also been proposed to act as α-secretase for amyloid precursor protein (APP) (6), and to be involved in the renewal and proliferation of neural stem cells (7).

1. Black, R.A. et al. (1997) Nature 385, 729-33.
2. Amin, A.R. (1999) Osteoarthritis Cartilage 7, 392-4.
3. Patel, I.R. et al. (1998) J Immunol 160, 4570-9.
4. Kenny, P.A. (2007) Differentiation 75, 800-8.
5. Delwig, A. and Rand, M.D. (2008) Cell Mol Life Sci 65, 2232-43.
6. Deuss, M. et al. (2008) Curr Alzheimer Res 5, 187-201.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody