Bmi1 (D20B7) XP? Rabbit mAb

• 产品编号：CST-6964P      品牌：Cell Signaling Technology       原厂货号：6964P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bmi1 (D20B7) XP® Rabbit mAb检测内源性Bmi1总蛋白。通过重组特异的人源Bmi1蛋白的羧基端去免疫动物从而制备出此单克隆抗体。polycomb group (PcG)蛋白有助于细胞身份的维持、干细胞自我更新、细胞周期调节和肿瘤形成，这些功能通过维持一些基因的沉默，而这些基因是促进细胞系特异性、细胞死亡和细胞周期停滞(1-4)。PcG蛋白存在两个复合物，它们配合通过表观遗传染色质修饰去维持长期的基因沉默。通过DNA结合转录因子和组蛋白H3在Lys27位点的甲基化，第一个复合物EED-EZH2被招募到基因上。这个组蛋白甲基转移酶活性需要该复合物的Ezh2、Eed和Suz12亚基(5)。组蛋白H3在Lys27位点的甲基化促进第二个复合物PRC1的招募，其能使组蛋白H2A在Lys119位点泛素化(6)。Bmi1蛋白是PRC1复合物的一个组成成分，该蛋白可与Ring1一起强有力的提高了Ring2催化亚基的E3泛素连接酶活性(7)。Bmi1蛋白在调节细胞增殖和衰老中起到重要作用，这主要通过p16 INK4A和p19 ARF基因的抑制，并且Bmi1蛋白对于成人造血和神经干细胞的维持是必须的(3,4,8-10)。

1. Boyer, L.A. et al. (2006) Nature 441, 349-53.
2. Lee, T.I. et al. (2006) Cell 125, 301-13.
3. Park, I.K. et al. (2003) Nature 423, 302-5.
4. Molofsky, A.V. et al. (2003) Nature 425, 962-7.
5. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
6. Wang, H. et al. (2004) Nature 431, 873-8.
7. Cao, R. et al. (2005) Mol Cell 20, 845-54.
8. Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7.
9. Jacobs, J.J. et al. (1999) Nature 397, 164-8.
10. Jacobs, J.J. et al. (1999) Genes Dev 13, 2678-90.

原厂资料：

Specificity / Sensitivity

Bmi1 (D20B7) XP® Rabbit mAb recognizes endogenous levels of total Bmi1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Bmi1 protein.

Background

The polycomb group (PcG) of proteins contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyl-transferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (6). Bmi1 is a component of the PRC1 complex, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit (7). Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and is required for maintenance of adult hematopoietic and neural stem cells (3,4,8-10).

1. Boyer, L.A. et al. (2006) Nature 441, 349-53.
2. Lee, T.I. et al. (2006) Cell 125, 301-13.
3. Park, I.K. et al. (2003) Nature 423, 302-5.
4. Molofsky, A.V. et al. (2003) Nature 425, 962-7.
5. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
6. Wang, H. et al. (2004) Nature 431, 873-8.
7. Cao, R. et al. (2005) Mol Cell 20, 845-54.
8. Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7.
9. Jacobs, J.J. et al. (1999) Nature 397, 164-8.
10. Jacobs, J.J. et al. (1999) Genes Dev 13, 2678-90.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.