GIT2 Antibody

• 产品编号：CST-6953S      品牌：CST       原厂货号：6953S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GIT2抗体识别内源性总GIT2蛋白水平。多抗由合成的肽段免疫动物产生，该合成肽段对应人GIT2蛋白的C末端附近残基。抗体由蛋白A和肽段亲和层析技术纯化。G蛋白偶联受体（GPCR）激酶相互作用蛋白1和2（GIT1和GIT2）是高度保守，广泛存在的scaffold蛋白，涉及局限的信号通路帮助调控粘着斑组装和细胞骨架动力学。GIT蛋白含多个相互作用结构域，允许与小GTP酶（包括ARF, Rac,和cdc42），激酶（如PAK和MEK），Rho家族GEF Pix,和焦点粘着蛋白paxillin（综述1）。GIT1 和 GIT2有很多同样的特性，但至少十个独特的组织特异剪切变异体。GIT2显示在抑制粘着斑更新和膜突出（2,3）。粘着斑定位和paxillin对GIT2的结合，是通过FAK 和/或 Src对一个或更多酪氨酸位点(Tyr286, Tyr392, Tyr592)的磷酸化调控（4,5,6）。一旦在粘着斑，GIT2被认为在细胞极性，迁移中起重要作用，使得它是一个检测癌基因信号通路的兴趣蛋白（3,5,7）。

1. Hoefen, R.J. and Berk, B.C. (2006) J Cell Sci 119, 1469-75.
2. Premont, R.T. et al. (2000) J Biol Chem 275, 22373-80.
3. Frank, S.R. et al. (2006) EMBO J 25, 1848-59.
4. Brown, M.C. et al. (2005) Mol Biol Cell 16, 4316-28.
5. Yu, J.A. et al. (2009) Mol Biol Cell 20, 4706-19.
6. Yu, J.A. et al. (2010) Cell Adh Migr 4, 342-7.
7. Mazaki, Y. et al. (2006) Nat Immunol 7, 724-31.

原厂资料：

Specificity / Sensitivity

GIT2 Antibody recognizes endogenous levels of total GIT2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GIT2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

G protein-coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac, and cdc42), kinases (such as PAK and MEK), the Rho family GEF Pix, and the focal adhesion protein paxillin (reviewed in 1). GIT1 and GIT2 share many of the same properties but with at least ten distinct, tissue-specific splice variants. GIT2 has been shown to play an important role in inhibiting focal adhesion turnover and membrane protrusion (2,3). Focal adhesion localization and paxillin binding of GIT2 is regulated through phosphorylation at one or more tyrosine sites (Tyr286, Tyr392, Tyr592) by FAK and/or Src (4,5,reviewed in 6). Once at the focal adhesion, GIT2 is thought to play a key role in cell polarity and migration, making it a protein of interest in the investigation of oncogenic signaling pathways (3,5,7).

1. Hoefen, R.J. and Berk, B.C. (2006) J Cell Sci 119, 1469-75.
2. Premont, R.T. et al. (2000) J Biol Chem 275, 22373-80.
3. Frank, S.R. et al. (2006) EMBO J 25, 1848-59.
4. Brown, M.C. et al. (2005) Mol Biol Cell 16, 4316-28.
5. Yu, J.A. et al. (2009) Mol Biol Cell 20, 4706-19.
6. Yu, J.A. et al. (2010) Cell Adh Migr 4, 342-7.
7. Mazaki, Y. et al. (2006) Nat Immunol 7, 724-31.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody