Phospho-TBC1D1 (Ser700) Antibody

• 产品编号：CST-6929S      品牌：CST       原厂货号：6929S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-TBC1D1 (Ser700) Antibody用于检测在转染的700位丝氨酸磷酸化的TBC1D1蛋白。多克隆抗体是通过合成对应于鼠TBC1D1蛋白Ser700及邻近序列的肽段免疫动物获得，抗体经Protein A和肽亲和层析纯化。TBC1D1是AS160（1）的旁系同源蛋白，他们有着约50%的相似性（2）。TBC1D1可能是严重肥胖的一个致病基因（3）。它通过自身的GAP活性在Glut4转移过程中发挥作用（2,4）。研究表明，TBC1D1在骨胳肌中高度表达（1）。胰岛素，AICAR以及它的浓度可以调节TBC1D1在骨骼肌中的磷酸化（1）。已经发现，TBC1D1中有三个AMPK磷酸化位点（Ser231, Ser660, 和Ser700）和一个Akt磷酸化位点（Thr590）（5）。肌肉收缩和AICAR处理会增加Ser231, Ser660, 和Ser700的磷酸化水平，但不会影响Thr590。只有胰岛素会增加Thr590位点的磷酸化水平（5）。

1. Taylor, E.B. et al. (2008) J Biol Chem 283, 9787-96.
2. Roach, W.G. et al. (2007) Biochem J 403, 353-8.
3. Stone, S. et al. (2006) Hum Mol Genet 15, 2709-20.
4. Chavez, J.A. et al. (2008) J Biol Chem 283, 9187-95.
5. Vichaiwong, K. et al. (2010) Biochem J 431, 311-20.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

Phospho-TBC1D1 (Ser700) Antibody detects transfected levels of TBC1D1 protein when phosphorylated at Ser700.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser700 of mouse TBC1D1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

TBC1D1 is a paralog of AS160 (1) and both proteins share about 50% identity (2). TBC1D1 was shown to be a candidate gene for severe obesity (3). It plays a role in Glut4 translocation through its GAP activity (2,4). Studies indicate that TBC1D1 is highly expressed in skeletal muscle (1). Insulin, AICAR, and contraction directly regulate TBC1D1 phosphorylation in this tissue (1). Three AMPK phosphorylation sites (Ser231, Ser660, and Ser700) and one Akt phosphorylation site (Thr590) were identified (5). Muscle contraction or AICAR treatment increases phosphorylation on Ser231, Ser660, and Ser700 but not on Thr590; insulin increases phosphorylation on Thr590 only (5).

1. Taylor, E.B. et al. (2008) J Biol Chem 283, 9787-96.
2. Roach, W.G. et al. (2007) Biochem J 403, 353-8.
3. Stone, S. et al. (2006) Hum Mol Genet 15, 2709-20.
4. Chavez, J.A. et al. (2008) J Biol Chem 283, 9187-95.
5. Vichaiwong, K. et al. (2010) Biochem J 431, 311-20.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody