Mena (D33C1) Rabbit mAb

• 产品编号：CST-6921S      品牌：CST       原厂货号：6921S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Mena (D33C1) Rabbit mAb 兔单抗识别内源性的总Mena蛋白。该单克隆抗体由合成的人类Mena蛋白Gly547位点附近肽段免疫动物而制成。Mena（哺乳动物激活的），EVL和VASP是ENA/ VASP家族的成员，它们通过从封顶蛋白中屏蔽肌动蛋白丝来控制细胞形态和细胞运动（1）。ENA/ VASP蛋白有三个特异性的结构域：一个氨基末端EVH1结构域，控制蛋白质的定位；一个富含脯氨酸的中央结构域，调节其与含SH3和WW结构域的蛋白质的相互作用，包括profilin；一个羧基末端结构域，用来组装成四聚体和与肌动蛋白结合（2）。Mena在神经元生长末端的片状伪足和神经元生长锥尖处丝状伪足处与肌动蛋白纤维相互作用。Mena基因纯合敲除的小鼠新生时大脑半球皮质到皮质神经元投射出现错误（3）。Mena被PKA在Ser236磷酸化，从而促进片状伪足的形成和神经锥的生长（4）。？目前已知三种形式的Mena，分别为80、88和140 kD。相比140kD蛋白主要在神经细胞中表达，80kD蛋白表达广泛。不同的剪切产生这些变体。88kD蛋白主要在胚胎细胞中发现，可能是翻译后修饰的产物。在Mena纯合子变异的动物中，三种形式的亚型都会消失（1,3）。

1. Gertler, F.B. et al. (1996) Cell 87, 227-39.
2. Small, J.V. (2008) Nat Cell Biol 10, 118-20.
3. Lanier, L.M. et al. (1999) Neuron 22, 313-25.
4. Lebrand, C. et al. (2004) Neuron 42, 37-49.

原厂资料：

Specificity / Sensitivity

Mena (D33C1) Rabbit mAb recognizes endogenous levels of total Mena protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly547 of human Mena protein.

Background

Mena (mammalian enabled), EVL, and VASP are members of the Ena/VASP family, which is involved in controlling cell shape and cell movement by shielding actin filaments from capping proteins (1). Ena/VASP proteins have three specific domains: an amino-terminal EVH1 domain controlling protein localization; a central proline-rich domain mediating interactions with both SH3 and WW domain containing proteins, including profilin; and a carboxy-terminal domain causing tetramerization and binding to actin (2). Mena interacts with actin filaments at the growing ends localizing to lamellipodia and to tips of growth cone filopodia in neurons. Axons projecting from interhemispheric cortico-cortical neurons are misrouted in newborn, homozygous Mena knock-out mice (3). Mena is phosphorylated at Ser236 by PKA, thereby promoting filopodial formation and elongation in the growth cone (4). 
 Three forms of Mena corresponding to 80, 88 and 140 kD are known. The 80 kD protein is broadly expressed in contrast to the 140 kD protein which is enriched in neural cell types. Alternative splicing produces these forms. The 88 kD protein is mainly found in embryonic cell types and is likely the result of post-translational modification. Expression of all three forms is completely eliminated in Mena homozygous mutant animals (1, 3).

1. Gertler, F.B. et al. (1996) Cell 87, 227-39.
2. Small, J.V. (2008) Nat Cell Biol 10, 118-20.
3. Lanier, L.M. et al. (1999) Neuron 22, 313-25.
4. Lebrand, C. et al. (2004) Neuron 42, 37-49.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody