SIK2 (D28G3) Rabbit mAb

• 产品编号：CST-6919S      品牌：CST       原厂货号：6919S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SIK2 (D28G3) Rabbit mAb兔单抗用于检测内源性SIK2总蛋白水平。该单抗是通过合成对应于人SIK2蛋白氨基端序列的肽段免疫动物获得。盐诱导激酶1（SIK1）最早在高盐摄食的大鼠肾上腺皮质中作为一种丝氨酸/苏氨酸激酶被发现（1）。SIK1是SNF1/AMPK家族中的一员，可以实现自我磷酸化（1）。SNF2是SIK1的一种同工酶，主要在脂肪组织中表达，在脂肪细胞分化时被诱导（2）。研究表明SIK2可以在Ser794位磷酸化人胰岛素受体底物（IRS－1）。已经有证据显示，在糖尿病小鼠的脂肪组织中，SIK2的表达及活性升高，这些发现可能说明SIK2在调控脂肪细胞中的胰岛素信号和胰岛素抵抗的发展过程中起着某些作用（2,3）。在空腹进食后，胰岛素可以诱导Akt2介导的SIK2蛋白在Ser358上的磷酸化，进而激活SIK2的激酶活性（4）。激活的SIK2然后诱导Torc2在Ser171上的磷酸化，这进一步引起辅转录因子从核转位到细胞质中，在那里被泛素化降解，从而抑制了糖异生基因的表达（4）。

1. Wang, Z. et al. (1999) FEBS Lett 453, 135-9.
2. Horike, N. et al. (2003) J Biol Chem 278, 18440-7.
3. Katoh, Y. et al. (2004) Mol Cell Endocrinol 217, 109-12.
4. Dentin, R. et al. (2007) Nature 449, 366-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

SIK2 (D28G3) Rabbit mAb recognizes endogenous levels of total SIK2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SIK2 protein.

Background

Salt-inducible kinase 1 (SIK1) was originally identified as a serine/threonine kinase from adrenocortical tissues of rats on a high salt diet (1). SIK1 is a SNF1/AMPK family kinase capable of autophosphorylation (1). SIK2 is an isoform of SIK1 and is specifically expressed in adipose tissues where it is induced during adipocyte differentiation (2). Studies suggest that SIK2 can phosphorylate human insulin receptor substrate (IRS-1) at Ser794. Along with evidence that SIK2 expression and activity are increased in white adipocytes of diabetic mice, this finding suggests a possible role for SIK2 in regulating insulin signaling in adipocytes and in the development of insulin resistance (2,3). Insulin triggers Akt2-mediated phosphorylation of SIK2 at Ser358 and the resultant kinase activation during post-fasting feeding (4). The activated SIK2 then induces the phosphorylation of Torc2 at Ser171 resulting in translocation of this transcriptional coactivator from the nucleus to cytoplasm where it is degraded through the ubiquitin pathway, leading to inhibition of gluconeogenic gene expression (4).

1. Wang, Z. et al. (1999) FEBS Lett 453, 135-9.
2. Horike, N. et al. (2003) J Biol Chem 278, 18440-7.
3. Katoh, Y. et al. (2004) Mol Cell Endocrinol 217, 109-12.
4. Dentin, R. et al. (2007) Nature 449, 366-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody