Bassoon (D63B6) Rabbit mAb

• 产品编号：CST-6897S      品牌：CST       原厂货号：6897S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bassoon (D63B6) Rabbit mAb 兔单抗识别内源性的总Bassoon蛋白。共聚焦免疫荧光分析观察到肌肉组织和脉络丛中有一些背景染色。该单克隆抗体由人类 bassoon蛋白中Gln1217位点附近肽段免疫动物而制成。Bassoon (BSN)，为了强调推测中其在突触囊泡循环中扮演的事件编排角色而命名（1），它是很大的卷曲螺旋蛋白，是兴奋性突触和抑制性突触细胞质活化区域中的核心组成成分（2）。BSN是突触带成分中的一种骨架蛋白，在活化区域形成电子致密结构以锚定突触囊泡。BSN在遗传上的异常会改变感光细胞和耳蜗内毛细胞中活化区域的锚定带（3），损害突触传递。这意味着BSN的存在对囊泡循环非常重要（4）。

1. Winter, C. et al. (1999) Genomics 57, 389-97.
2. Hallermann, S. et al. (2010) Neuron 68, 710-23.
3. Frank, T. et al. (2010) Neuron 68, 724-38.
4. Joselevitch, C. and Zenisek, D. (2010) Neuron 68, 604-6.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

Bassoon (D63B6) Rabbit mAb recognizes endogenous levels of total Bassoon protein. Some background staining was observed in muscle tissue and the choroid plexus by confocal immunofluorescent analysis.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln1217 of human bassoon protein.

Background

Bassoon (BSN), named such to underline its presumptive role in orchestrating events of the synaptic vesicle cycle (1), is a very large coiled-coil protein and is one of the core components of the cytomatrix at the active zones of both excitatory and inhibitory synapses (2). BSN is a scaffold protein that is a component of the synaptic ribbon, an electron-dense structure anchored at the active zone that tethers synaptic vesicles. Genetic disruption of BSN displaces the anchoring of ribbons to the active zones of photoreceptors and cochlear inner hair cells (3), and this displacement of the ribbons substantially impairs synaptic transmission, suggesting that, when present, BSN is important for the vesicle cycle (4).

1. Winter, C. et al. (1999) Genomics 57, 389-97.
2. Hallermann, S. et al. (2010) Neuron 68, 710-23.
3. Frank, T. et al. (2010) Neuron 68, 724-38.
4. Joselevitch, C. and Zenisek, D. (2010) Neuron 68, 604-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody