α-Actinin (D6F6) XP? Rabbit mAb

• 产品编号：CST-6487P      品牌：Cell Signaling Technology       原厂货号：6487P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

α-Actinin (D6F6) XP®Rabbit mAb可以识别内源性总的α-actinin 蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人α-actinin蛋白316位苯丙氨酸附近氨基酸一致。α-Actinin属于细胞骨架蛋白pectrin家族。它最早被发现作为actin交联蛋白，形成反向同二聚体，互相结合在氨基末端。最近，α-actinin被发现可以与大量的涉及细胞骨架信号通路的蛋白相结合，包括那些涉及到细胞黏附，迁移和免疫细胞应激的过程（1）。α-actinin与细胞间黏附分子-5（ICAM-5）帮助促进神经突出的增生（2）。在成骨细胞中，α-actinin和integrins结合稳定黏着斑可能会保护细胞不发生凋亡（3）。α-actinin的1和4亚型，是非肌肉亚型，在压力纤维、黏附和细胞间联系，丝状伪足和板状伪足的部位被发现。肌肉亚型2和3位于肌肉的Z显带和平滑肌的致密斑（1）。

1. Otey, C.A. and Carpen, O. (2004) Cell Motil Cytoskeleton 58, 104-11.
2. Nyman-Huttunen, H. et al. (2006) J Cell Sci 119, 3057-66.
3. Triplett, J.W. and Pavalko, F.M. (2006) Am J Physiol Cell Physiol 291, C909-21.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Zebrafish

Specificity / Sensitivity

α-Actinin (D6F6) XP® Rabbit mAb recognizes endogenous levels of total α-actinin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe316 of human α-actinin protein.

Background

α-Actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, α-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration, and immune cell targeting (1). The interaction of α-actinin with intercellular adhesion molecule-5 (ICAM-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of α-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of α-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia, and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).

1. Otey, C.A. and Carpen, O. (2004) Cell Motil Cytoskeleton 58, 104-11.
2. Nyman-Huttunen, H. et al. (2006) J Cell Sci 119, 3057-66.
3. Triplett, J.W. and Pavalko, F.M. (2006) Am J Physiol Cell Physiol 291, C909-21.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.