REPS1 (D6F4) Rabbit mAb

• 产品编号：CST-6404S      品牌：CST       原厂货号：6404S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

REPS1 (D6F4) Rabbit mAb兔单抗识别内源性的REPS1总蛋白蛋白水平。该单克隆抗体用与人类REPS1蛋白中Asn189位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。REPS1是一个RalBP1结合的包含EH同源结构域的蛋白。REPS1序列具有一个EH结构域，随后有两个富含脯氨酸的区域，和一个羧基末端的卷曲螺旋结构域，用以结合RalBP1（1）。REPS1的EH区域与Rab11-FIP2的NPF基序相互作用，介导它们在囊泡上共区域化，影响EGFR的内吞（2）。REPS1的两个脯氨酸富集区对结合GRK/GRB2的SH3区域十分重要，并且可以进一步调节EGFR下游信号。REPS1的脯氨酸富集区也显示可与intersectin1(ITSN1)的SH3区域相互作用，对ITSN1/SGIP1/REPS1复合物在网格蛋白包被坑中的形成有贡献。三种不同REPS1的剪接形式已经被确定。

1. Yamaguchi, A. et al. (1997) J Biol Chem 272, 31230-4.
2. Cullis, D.N. et al. (2002) J Biol Chem 277, 49158-66.
3. Dergai, O. et al. (2010) Biochem Biophys Res Commun 402, 408-13.

原厂资料：

Specificity / Sensitivity

REPS1 (D6F4) Rabbit mAb recognizes endogenous levels of total REPS1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn189 of human REPS1 protein.

Background

REPS1 is a RalBP1-associated EH-homology domain containing protein. The sequence of REPS1 has an EH domain, followed by two proline-rich segments, and a C-terminal coiled-coil domain for binding to RalBP1 (1). The EH domain of REPS1 interacts with the NPF motif of Rab11-FIP2, mediates their colocalization to endosome vesicles, and influences EGFR endocytosis (2). The two proline-rich regions of REPS1 are important for binding to the SH3 domain of GRK/GRB2 and further regulate EGFR downstream signaling. The proline-rich regions of REPS1 have also been shown to interact with the SH3 domain of intersectin1 (ITSN1) and contribute to ITSN1/SGIP1/REPS1 complex formation on clathrin-coated pits. Three alternatively spliced isoforms of REPS1 have been identified.

1. Yamaguchi, A. et al. (1997) J Biol Chem 272, 31230-4.
2. Cullis, D.N. et al. (2002) J Biol Chem 277, 49158-66.
3. Dergai, O. et al. (2010) Biochem Biophys Res Commun 402, 408-13.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody