ACF1 Antibody

• 产品编号：CST-6255S      品牌：CST       原厂货号：6255S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ACF1 Antibody检测内源性ACF1总蛋白(isoforms 1和2)。通过人工合成人源ACF1蛋白Met864位点周围相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。哺乳动物imitation SWI (ISWI)复合物是以两个ATPase亚单位Snf2h和Snf2l 为特征(1)。Snf2h与ATP-utilizing chromatin assembly and remodeling factor 1 (ACF1)相互作用，这包含了ACF染色质重塑复合物(1)。ACF1 (BAZ1A)在发育(2)、染色质结构的调节 (3)和DNA损伤反应(4,5)中有明显作用。在果蝇中不同的发育阶段指示ACF1的表达，并且在果蝇发育期间ACF1表达的改变导致脱离正常染色质组织(2)。在发育阶段ACF1蛋白在异源染色质形成中有作用，并且涉及多种染色质结构的起初构建。在体内研究证明heterochromatin protein 1 (HP1)结合到histone H3蛋白甲基化的lysine 9，该过程是通过与染色质一起ACF1的相互作用被提高的(6)。在DNA损伤期间染色质重塑因子是被需要的，这是为了允许信号分子和损伤激酶去达到该位点(4)。hACF1的损耗增加了凋亡和在X-射线和UV照射下易受辐射以及G2/M期停滞的激活(4)。ACF1蛋白损耗也敏感于DNA double-stranded breaks (DSBs)的细胞，并且损伤DNA修复(5)。尤其在DSBs位点Ku的堆积可能依赖于ACF1蛋白的存在(5)。

1. Saladi, S.V. and de la Serna, I.L. (2010) Stem Cell Rev 6, 62-73.
2. Chioda, M. et al. (2010) Development 137, 3513-22.
3. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
4. Sánchez-Molina, S. et al. (2011) Nucleic Acids Res 39, 8445-56.
5. Lan, L. et al. (2010) Mol Cell 40, 976-87.
6. Eskeland, R. et al. (2007) Mol Cell Biol 27, 453-65.

原厂资料：

Specificity / Sensitivity

ACF1 Antibody recognizes endogenous levels of total ACF1 protein (isoforms 1 and 2).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met864 of human ACF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The mammalian imitation SWI (ISWI) complexes are characterized by two ATPase subunits: Snf2h and Snf2l (1). Snf2h interacts with ATP-utilizing chromatin assembly and remodeling factor 1 (ACF1) to comprise the ACF chromatin-remodeling complex (1). ACF1 (BAZ1A) has distinct roles in development (2), regulation of chromatin structure (3), and DNA damage response (4,5). Different developmental stages dictate the expression of ACF1 in Drosophila, and alterations in ACF1 expression during Drosophila development leads to deviation from normal chromatin organization (2). ACF1 functions in heterochromatin formation during development and is involved in the initial establishment of diversified chromatin structures. In vivo studies demonstrate that heterochromatin protein 1 (HP1) binding to methylated lysine 9 of histone H3 is enhanced by the interaction of ACF1 with chromatin (6). Chromatin-remodeling factors are required during DNA damage in order to allow signaling molecules and damaging enzymes to access the site (4). Depletion of hACF1 increases apoptosis and vulnerability to radiation and compromises G2/M arrest activated in response to X-ray and UV exposure (4). Depletion of ACF1 also sensitizes cells to DNA double-stranded breaks (DSBs) and impairs DNA repair (5). Specifically, accumulation of Ku at DSBs sites may depend on the presence of ACF1 (5).

1. Saladi, S.V. and de la Serna, I.L. (2010) Stem Cell Rev 6, 62-73.
2. Chioda, M. et al. (2010) Development 137, 3513-22.
3. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
4. Sánchez-Molina, S. et al. (2011) Nucleic Acids Res 39, 8445-56.
5. Lan, L. et al. (2010) Mol Cell 40, 976-87.
6. Eskeland, R. et al. (2007) Mol Cell Biol 27, 453-65.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.