AIF (D39D2) XP? Rabbit mAb (Sepharose? Bead Conjugate)

• 产品编号：CST-5939S      品牌：CST       原厂货号：5939S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AIF (D39D2) XP^® Rabbit mAb (Sepharose Bead Conjugate) 兔单抗能够检测内源性水平AIF总蛋白。该单克隆抗体是由合成的人源AIF蛋白Ala520周围序列相对应的肽段免疫动物而制备的。该CST抗体通过伯氨基基团共价结合固定到N-羟基琥珀酰亚胺（NHS）活化的琼脂糖磁珠。AIF (D39D2) XP^® Rabbit mAb (Sepharose Bead Conjugate)兔单抗可用于AIF蛋白的免疫沉淀试验。该抗体与不带标记的AIF (D39D2) XP^® Rabbit mAb兔单抗#5318有着相同的种属交叉反应。凋亡诱导因子(AIF, PDCD8)广泛表达黄素蛋白，在caspase依赖的凋亡中起着至关重要的作用（见综述1,2）。 AIF通常定位于线粒体的间隙，应答凋亡刺激而释放（3）。用重组AIF蛋白处理分离的细胞核会导致早期凋亡现象，如染色质浓缩，大规模的DNA片段化（3）。通过针对AIF基因敲除小鼠的研究表明，AIF的凋亡活性跟细胞类型和刺激有关。另据报道，AIF与胚体空化相关，相当于胚胎形态发生过程中程序性细胞死亡的第一波（4）。AIF的结构分析揭示了两个重要的区域，一个具有氧化还原酶活性，另一个是潜在的DNA结合结构域（3,5）。AIF有氧化还原活性，可以表现为NADH氧化酶，但在诱导细胞凋亡过程中并不需要这种活性（6）。然而，最近的研究表明AIF具有双重功能，通过在细胞核中结合DNA发挥促凋亡活性，以及通过其氧化还原酶活性清除自由基发挥抗凋亡活性（2,7）。

1. Daugas, E. et al. (2000) FEBS Lett 476, 118-23.
2. Lipton, S.A. and Bossy-Wetzel, E. (2002) Cell 111, 147-50.
3. Susin, S.A. et al. (1999) Nature 397, 441-6.
4. Joza, N. et al. (2001) Nature 410, 549-54.
5. Ye, H. et al. (2002) Nat Struct Biol 9, 680-4.
6. Miramar, M.D. et al. (2001) J Biol Chem 276, 16391-8.
7. Klein, J.A. et al. (2002) Nature 419, 367-74.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog

Specificity / Sensitivity

AIF (D39D2) XP^® Rabbit mAb (Sepharose Bead Conjugate) recognizes endogenous levels of total AIF protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to redidues surrounding Ala520 of human AIF protein.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. AIF (D39D2) XP^® Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of AIF. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated AIF (D39D2) XP^® Rabbit mAb #5318.

Background

Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (reviewed in 1,2). AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimuli (3). Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation (3). Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis (4). Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain (3,5). While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis (6). Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity (2,7).

1. Daugas, E. et al. (2000) FEBS Lett 476, 118-23.
2. Lipton, S.A. and Bossy-Wetzel, E. (2002) Cell 111, 147-50.
3. Susin, S.A. et al. (1999) Nature 397, 441-6.
4. Joza, N. et al. (2001) Nature 410, 549-54.
5. Ye, H. et al. (2002) Nat Struct Biol 9, 680-4.
6. Miramar, M.D. et al. (2001) J Biol Chem 276, 16391-8.
7. Klein, J.A. et al. (2002) Nature 419, 367-74.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not
aliquot the antibody