GATA-3 (D13C9) XP? Rabbit mAb

• 产品编号：CST-5852S      品牌：CST       原厂货号：5852S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GATA-3 (D13C9)XP^® Rabbit mAb兔单抗识别内源性的总GATA-3蛋白 。单克隆抗体由合成的肽段免疫动物产生。其合成的肽段与邻近人源GATA-3蛋白氨基末端的氨基酸残基序列一致。GATA-3是在小鼠和人的各种系统的发育中存在的关键调控因子[4]。GATA-3缺失的小鼠胚胎会因大脑和脊柱的生长迟缓和变形并在E11和E12之间致死[5]。GATA-3的功能已在T细胞发育中专门研究，近来显示它在Notch调控的TH2细胞分化中是Notch下游的靶标[6,7]。它在造血和非造血组织中表达，包括肾脏、皮肤、乳腺和中枢神经系统[8-10]。在管腔乳腺癌总GATA-3的减少与不良临床预后相关。GATA-3的表达水平可能因此作为很有希望的愈后标志物[11]。GATA-3单倍剂量不足导致人的Barakat综合症，这是感觉神经耳聋和肾发育不良的条件特征之一[12]。

1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
4. Debacker, C. et al. (1999) Mech Dev 85, 183-7.
5. Pandolfi, P.P. et al. (1995) Nat Genet 11, 40-4.
6. Ho, I.C. et al. (2009) Nat Rev Immunol 9, 125-35.
7. Amsen, D. et al. (2007) Immunity 27, 89-99.
8. Grote, D. et al. (2008) PLoS Genet 4, e1000316.
9. Kaufman, C.K. et al. (2003) Genes Dev 17, 2108-22.
10. Kouros-Mehr, H. et al. (2006) Cell 127, 1041-55.
11. Chou, J. et al. (2010) J Cell Physiol 222, 42-9.
12. Van Esch, H. et al. (2000) Nature 406, 419-22.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

GATA-3 (D13C9) XP^® Rabbit mAb recognizes endogenous levels of total GATA-3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-3 protein.

Background

GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).GATA-3 is a critical regulator of development of various systems in both mouse and human (4). GATA-3 null mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (5). The function of GATA-3 has been extensively studied in T cell development and has recently been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (6,7). It is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (8-10). Decreased expression of GATA-3 in luminal breast cancer is associated with poor clinical outcome. GATA-3 expression level may therefore be a promising prognostic biomarker (11). Haploinsufficiency of GATA-3 results in Barakat syndome in humans, a condition characterized by sensorineural deafness and renal dysplasia (12).

1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
4. Debacker, C. et al. (1999) Mech Dev 85, 183-7.
5. Pandolfi, P.P. et al. (1995) Nat Genet 11, 40-4.
6. Ho, I.C. et al. (2009) Nat Rev Immunol 9, 125-35.
7. Amsen, D. et al. (2007) Immunity 27, 89-99.
8. Grote, D. et al. (2008) PLoS Genet 4, e1000316.
9. Kaufman, C.K. et al. (2003) Genes Dev 17, 2108-22.
10. Kouros-Mehr, H. et al. (2006) Cell 127, 1041-55.
11. Chou, J. et al. (2010) J Cell Physiol 222, 42-9.
12. Van Esch, H. et al. (2000) Nature 406, 419-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody