Sall4 Antibody

• 产品编号：CST-5850S      品牌：CST       原厂货号：5850S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Sall4抗体识别内源性的总Sall4蛋白亚型A和B，这种抗体也可检测其他Sall4亚型。多克隆抗体由合成的肽段免疫动物产生。其合成的肽段与人源Sall4蛋白羧基末端氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析法纯化。SALL基因家族成员编码发育过程中高表达的锌指转录因子（1）。Sall4与其他多潜能调控因子，例如Oct-4和Nanog，在发育的极早期就开始表达（2）。最近的研究提示Sall4作为主要调控因子以一种转录调控反馈机制控制了其自身和Oct-4的表达以控制干细胞多潜能性和干细胞命运（2,3）。免疫组化实验指出Sall4是初级生殖细胞瘤和卵黄囊瘤的一个敏感且特异的诊断标志（4,5）。研究显示Sall4在急性髓细胞性白血病（AML）中持续激活表达，是Wnt/β-catenin在该疾病中一个可能的效应因子（6）。除此以外，Sall4突变被发现可能涉及了人类畸形综合征，包括Duane-radial ray 综合症 (Okihiro syndrome)和Acro-renal-ocular 综合症 (7)。

1. Sweetman, D. and Münsterberg, A. (2006) Dev Biol 293, 285-93.
2. Yang, J. et al. (2008) Proc Natl Acad Sci USA 105, 19756-61.
3. Yang, J. et al. (2010) PLoS One 5, e10766.
4. Mei, K. et al. (2009) Mod Pathol 22, 1628-36.
5. Miertus, J. et al. (2006) Hum Genet 119, 154-61.
6. Ma, Y. et al. (2006) Blood 108, 2726-35.
7. Al-Baradie, R. et al. (2002) Am J Hum Genet 71, 1195-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Sall4 Antibody recognizes endogenous levels of total Sall4 protein, isoforms A and B. This antibody may also detect other Sall4 isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Sall4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Members of the SALL gene family encode putative zinc finger transcription factors highly expressed during development (1). Sall4 is expressed very early in development with other pluripotency regulators, such as Oct-4 and Nanog (2). Recent studies suggest Sall4 works as a master regulator that controls its own expression and the expression of Oct-4 in a transcriptional regulation feedback loop governing stem cell pluripotency and stem cell fate (2,3). Immunohistochemical studies indicate that Sall4 is a sensitive and specific diagnostic marker for primary germ cell tumors and yolk sac tumors (4,5). Research studies have shown that Sall4 is constitutively expressed in acute myeloid leukemia (AML) and is a probable effector of the Wnt/β-catenin signaling pathway in this disease (6). In addition, mutations in Sall4 have been implicated in human malformation syndromes including Duane-radial ray syndrome (Okihiro syndrome) and Acro-renal-ocular syndrome (7).

1. Sweetman, D. and Münsterberg, A. (2006) Dev Biol 293, 285-93.
2. Yang, J. et al. (2008) Proc Natl Acad Sci USA 105, 19756-61.
3. Yang, J. et al. (2010) PLoS One 5, e10766.
4. Mei, K. et al. (2009) Mod Pathol 22, 1628-36.
5. Miertus, J. et al. (2006) Hum Genet 119, 154-61.
6. Ma, Y. et al. (2006) Blood 108, 2726-35.
7. Al-Baradie, R. et al. (2002) Am J Hum Genet 71, 1195-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.