SDHA Antibody

• 产品编号：CST-5839S      品牌：CST       原厂货号：5839S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SDHA抗体用于检测内源性SDHA总蛋白水平。多克隆抗体是通过合成对应于人SDHA蛋白羧基端序列的肽段免疫动物获得，抗体经Protein A和肽亲和层析纯化。琥珀酸脱氢酶(SDH)，也称作复合体II和琥珀酸醌氧化还原酶，是柠檬酸循环和电子传递链的一个关键组分（1）。具体来说，它负责琥珀酸的氧化反应（2）。SDH由四个亚基组成，SDHA, SDHB, SDHC和 SDHD (3）。SDHA的缺陷引起复合体II功能缺失（2）。除此之外，在Huntington病人的纹状体中SDHA数量减少（3）。通常在副神经节瘤和 phenochromocytomas中，SDHB, SDHC和 SDHD的数量减少（4,5）。

1. Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31.
2. Bourgeron, T. et al. (1995) Nat Genet 11, 144-9.
3. Benchoua, A. et al. (2006) Mol Biol Cell 17, 1652-63.
4. Baysal, B.E. et al. (2000) Science 287, 848-51.
5. Feichtinger, R.G. et al. (2010) BMC Cancer 10, 149.

原厂资料：

Specificity / Sensitivity

SDHA Antibody recognizes endogenous levels of total SDHA protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SDHA protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Succinate dehydrogenase (SDH), also known as Complex II or succinate:quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (1). Specifically, it is involved in the oxidation of succinate (2). SDH consists of four subunits: SDHA, SDHB, SDHC, and SDHD (3). Defects in SDHA cause complex II deficiency (2). In addition, reduction of SDHA has been observed in the striatum of patients with Huntington’s Disease (3). Reduction of SDHB, SDHC, and SDHD are often observed in paragangliomas and phenochromocytomas (4,5).

1. Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31.
2. Bourgeron, T. et al. (1995) Nat Genet 11, 144-9.
3. Benchoua, A. et al. (2006) Mol Biol Cell 17, 1652-63.
4. Baysal, B.E. et al. (2000) Science 287, 848-51.
5. Feichtinger, R.G. et al. (2010) BMC Cancer 10, 149.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody