c-Kit (D13A2) XP? Rabbit mAb (Biotinylated)

• 产品编号：CST-5749S      品牌：CST       原厂货号：5749S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

c-Kit的抗体(D13A2) XP®(生物素偶联)能检测内源c-Kit蛋白的表达水平。本抗体不与其它酪氨酸激酶受体家族的成员发生交叉反应。单克隆抗体通过免疫动物得到，该多肽是根据人的c-Kit蛋白Tyr703附近的氨基酸序列合成的。本CST公司生产的抗体在最佳条件下与生物素偶联。该生物素偶联的抗体具有和未偶联生物素的c-Kit (D13A2) XP® Rabbit mAb #3074相同的物种交叉反应特性。c-Kit是受体酪氨酸激酶亚族的成员之一，这个家族包扩PDGF、 CSF-1和 FLT3/flk-2受体 (1,2)。它在造血干细胞、肥大细胞、黑色素细胞和生殖细胞等多种细胞的活化和生长过程中起重要作用(3)。通过和配体干细胞因子 (SCF)相结合，c-Kit发生二聚化/寡聚化并自磷酸化。激活的c-Kit能够招募下游含SH2信号传到成分，包括PLCγ、PI3K激酶的p85亚基、 SHP2和 CrkL，并使其酪氨酸磷酸化 (4)。导致c-Kit激酶功能减弱的分子损伤往往和各种发育异常相关(5)，而促使c-Kit组成型活化的突变则能导致肥大细胞增多症和胃肠道间质肿瘤(6)。Tyr719位于激酶催化插入区域。Tyr719位点磷酸化的c-Kit 的能够在体内或体外与PI3K激酶的p85亚基结合(7)。

1. Martin, F.H. et al. (1990) Cell 63, 203-11.
2. Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
3. Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
4. Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
5. Nocka, K. et al. (1990) EMBO J 9, 1805-13.
6. Hirota, S. et al. (1998) Science 279, 577-80.
7. Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.

原厂资料：

Specificity / Sensitivity

c-Kit (D13A2) XP® Rabbit mAb (Biotinylated) detects endogenous levels of total c-Kit protein. It does not cross-react with other receptor tyrosine kinase family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr703 of human c-Kit protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated c-Kit (D13A2) XP® Rabbit mAb #3074. 

Background

c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).

1. Martin, F.H. et al. (1990) Cell 63, 203-11.
2. Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
3. Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
4. Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
5. Nocka, K. et al. (1990) EMBO J 9, 1805-13.
6. Hirota, S. et al. (1998) Science 279, 577-80.
7. Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot antibody.