MEK1 (61B12) Mouse mAb (Biotinylated)

• 产品编号：CST-5744S      品牌：CST       原厂货号：5744S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

MEK1 (61B12) Mouse mAb (Biotinylated)鼠单抗能够检测内源性MEK1总蛋白水平。该抗体不会与 MEK2和其他MAPK的激酶发生交叉反应。该单克隆抗体是采用全长MEK1蛋白免疫动物后产生的。Cell Signaling Technology的生物素偶联的抗体都是在优化条件下生产的。此生物素偶联的抗体预期和未偶联的MEK1 (61B12) Mouse mAb #2352鼠单抗有相同的物种交叉反应性。MEK1、MEK2,也被称为MAPK或Erk激酶，它们是双特异性蛋白激酶，在丝裂原活化蛋白激酶级联反应中发挥作用，控制细胞生长和分化(1-3)。MEK1、MEK2的活化是通过Raf样分子对两个丝氨酸（217 /221位点）的磷酸化而实现的，这两个丝氨酸位点位于亚结构域VIII的活化环内。多种生长因子和细胞因子以及膜去极化和钙内流都能激活MEK1/2(1-4)。组成型活化的MEK1/2能够诱导NIH/3T3细胞的转化和PC-12细胞的分化(4)。MEK蛋白通过将p44、p42 MAPK激酶上位于亚结构域VIII的活化环内的丝氨酸和苏氨酸磷酸化，激活p44、 p42 MAPK激酶。

1. Crews, C.M. et al. (1992) Science 258, 478-480.
2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
3. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
4. Cowley, S. et al. (1994) Cell 77, 841-852.

原厂资料：

Specificity / Sensitivity

MEK1 (61B12) Mouse mAb (Biotinylated) recognizes endogenous levels of total MEK1 protein. This antibody does not cross-react with MEK2 and other MAP kinase kinases.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full length MEK1 proteins.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated MEK1 (61B12) Mouse mAb #2352.

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

1. Crews, C.M. et al. (1992) Science 258, 478-480.
2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
3. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
4. Cowley, S. et al. (1994) Cell 77, 841-852.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot antibody.