WNK4 Antibody

• 产品编号：CST-5713S      品牌：CST       原厂货号：5713S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

WNK4 Antibody可以检测内源性总WNK4蛋白。多克隆抗体是采用人类WNK4蛋白Lys59周围残基相对应的合成肽段免疫动物生产的。抗体采用蛋白A和肽亲和层析法来纯化。WNK[无赖氨酸(K)]丝氨酸和苏氨酸激酶家族的特点是其激酶活性域的子域II 赖氨酸被半胱氨酸取代（1、2）。赖氨酸对于磷酰基转移时必需的，在催化域的非典型位置。4个WNK家族成员已经在人类被确认(WNK1-4)，参与离子通透（3）。人类WNK1 和 WNK4基因的突变可以引起假性醛固酮减少症类型II (PHAII), 是常染色体显性遗传病，可以引起高血压，高钾血症和肾小管硬化（4）， WNK4特异性表达在肾脏，而WNK1则广泛表达，主要表达在极化的上皮细胞上（1-3）。小鼠WNK1的杂合突变引起血压的显著降低，然而纯合突变会引起胚胎死亡（5）. WNK1在Thr60位点被Akt磷酸化（6）。另外， WNK1 在活化畔中在Ser382位点发生自磷酸化，被认为是激酶活性必须（7）。

1. Veríssimo, F. and Jordan, P. (2001) Oncogene 20, 5562-9.
2. Xu, B. et al. (2000) J Biol Chem 275, 16795-801.
3. Choate, K.A. et al. (2003) Proc Natl Acad Sci U S A 100, 663-8.
4. Wilson, F.H. et al. (2001) Science 293, 1107-12.
5. Zambrowicz, B.P. et al. (2003) Proc Natl Acad Sci U S A 100, 14109-14.
6. Vitari, A.C. et al. (2004) Biochem J 378, 257-68.
7. Xu, B.E. et al. (2002) J Biol Chem 277, 48456-62.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

WNK4 Antibody detects endogenous levels of total WNK4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a sythetic peptide corresponding to residues surrounding Lys59 of human WNK4. Antibodies were purified by protein A and peptide affinity chromatography.

Background

The WNK [with no lysine (K)] family of serine/threonine kinases is characterized by having a cysteine in place of lysine in subdomain II of its kinase activation domain (1,2). The lysine necessary for phosphoryl transfer is located in an atypical position in the catalytic domain. Four WNK family members have been identified in humans (WNK1-4) and have been implicated in regulating ion permeability (3). Mutations in the WNK1 and WNK4 genes in humans cause pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder leading to hypertension, hyperkalemia, and renal tubular acidosis (4). WNK4 is specifically expressed in the kidney, whereas WNK1 has a wider distribution but is predominantly expressed in polarized epithelia (1-3). Heterozygous mutations in WNK1 in mice result in a significant decrease in blood pressure, while homozygous mutations are embryonic lethal (5). WNK1 is phosphorylated by Akt at Thr60 (6). In addition, WNK1 may be autophosphorylated at Ser382 in the activation loop, and this is thought to be required for its kinase activity (7).

1. Veríssimo, F. and Jordan, P. (2001) Oncogene 20, 5562-9.
2. Xu, B. et al. (2000) J Biol Chem 275, 16795-801.
3. Choate, K.A. et al. (2003) Proc Natl Acad Sci U S A 100, 663-8.
4. Wilson, F.H. et al. (2001) Science 293, 1107-12.
5. Zambrowicz, B.P. et al. (2003) Proc Natl Acad Sci U S A 100, 14109-14.
6. Vitari, A.C. et al. (2004) Biochem J 378, 257-68.
7. Xu, B.E. et al. (2002) J Biol Chem 277, 48456-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.